Method for determining anthocyanin composition spectrum and content in germplasm and seed coat of black soyabean by utilizing HPLC

Abstract

The invention relates to a method for determining anthocyanin compostion spectrum and content in germplasm and seed coat of black soyabean by utilizing HPLC, including the following steps: (1) black soyabean sample solution is prepared; (2) chromatograph condition is determined; (2) according to the chromatograph condition in the step (2), the black soyabean sample solution and standard solution are injected into high performance liquid chromatograph, and composition spectrum of each anthocyanin is obtained by comparing preservation time of each anthocyanin and the standard solution and adopting labelling and quantitative method; (4) according to the chromatograph condition in the step (2), the black soyabean sample solution is injected into high performance liquid chromatograph, and the content of each anthocyanin in germplasm and seed coat of black soyabean is calculated by adopting standard curve method. The method has the characteristics of accuracy, high sensibility and good repeatability; the defects that only total content of anthocyanin can be determined in the previous method for determining anthocyanin content by a spectrophotometer by pH differential method and the result is poor in accuracy are avoided; and components and content of each anthocyanin in seed coat of black soyabean can be determined.

Description

technical field [0001] The invention relates to a method for measuring the composition spectrum and content of black soybean germplasm seed coat anthocyanins by using HPLC, and belongs to the technical field of liquid chromatography analysis. Background technique [0002] Anthocyanins are a class of water-soluble pigments widely present in the seed coats and peels of various plants in nature, and have significant biological activities such as anti-oxidation, anti-tumor, and vision improvement. Medical research shows that the intake of anthocyanins is significantly negatively correlated with the morbidity and mortality of many chronic diseases such as cardiovascular and cerebrovascular diseases and cancer. At present, anthocyanins have become an important functional ingredient in the development of cosmetics and various health foods. Therefore, looking for plant sources with high anthocyanin content, used to guide and improve people's dietary structure and as a raw material ...

AI Technical Summary

Problems solved by technology

[0003] Based on the fact that anthocyanin compounds have characteristic absorption peaks between 505-530nm, UV-Vis spectroscopy is often used to measure the total anthocyanin content at present. It is only suitable for large-scale preliminary screening; the determination of anthocyanins by HPLC method can overcome the above shortcomings, but the current common detection method requires a complex anthocyanin In the extraction and purification process, the pretreatment work is cumbersome, which greatly increases the workload of the determination

At the same time, the multi-step separation and purification process will inevitably lose some target substances. Without a high enough sample processing capacity, some anthocyanin components with very low content are difficult to be detected.

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