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Lactobacillus mucosae capable of resisting high-concentration butanol

A Lactobacillus mucosae, high-concentration technology, applied in the field of bioengineering, can solve the problem of low butanol tolerance, and achieve the effect of broadening the selection of species

Inactive Publication Date: 2010-05-26
GUANGXI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Escherichia coli K12 in the genus Escherichia coli can tolerate a maximum butanol concentration of 8 g / L because Escherichia coli itself is not very tolerant to butanol, so it is not suitable for large-scale industrial production of butanol

Method used

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  • Lactobacillus mucosae capable of resisting high-concentration butanol
  • Lactobacillus mucosae capable of resisting high-concentration butanol

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Isolation, screening and identification of a Lactobacillus mucosa strain BTPZ 14-14 that can tolerate high concentration of butanol.

[0020] Step 1: Sampling

[0021] Samples were collected from compost in the suburbs of Nanning City, Guangxi Zhuang Autonomous Region.

[0022] The second step: primary screening

[0023] Ordinary medium (g / L): peptone 10.0, beef extract 8.0, yeast extract 4.0, glucose 20.0, sodium acetate 5.0, Tween 801.0, diammonium hydrogen citrate 2.0, dipotassium hydrogen phosphate 2.0, MgSO 4 ·7H 2 O 0.2, MnSO 4 ·H 2 O 0.05, pH 6.2.

[0024] Screening medium I: Add 20 g / L n-butanol to the normal medium.

[0025] Screening medium II: add 25g / L n-butanol and 20g / L agar to ordinary medium.

[0026] Screening medium III: Add 25g / L-30g / L n-butanol to ordinary medium.

[0027] Disperse the collected samples with sterile water, put them into a test tube containing 30 mL of screening medium I, and culture them at a constant temperature of 37°C for ...

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Abstract

The invention relates to Lactobacillus mucosae capable of resisting high-concentration butanol. The bacterial strain is Lactobacillus mucosae BTPZ 14-14, and the preservation number of the strain is CCTCC No.M209207. The invention obtains a bacterial strain capable of resisting 30g / L of butanol by screening microbes capable of resisting butanol in the natural environment, and the bacterial strain is facultative anaerobe capable of utilizing multiple carbon sources such as xylose, glucose, sucrose and the like.

Description

technical field [0001] The invention relates to the technical field of bioengineering. In particular, a strain of Lactobacillus mucosae BTPZ 14-14 can tolerate high concentrations of butanol. Background technique [0002] Bio-butanol is a new generation of bio-energy with great application value. With the reduction of oil resources, it has become the consensus of all countries to develop a new generation of fuels, diversify the fuel structure, and reduce the dependence on oil resources. The preparation of liquid fuels from various renewable biomass, such as ethanol and butanol, is a feasible new energy solution to replace petroleum. [0003] Butanol has the characteristics of high energy density, low hydrophilicity, high octane number and low volatility. Compared with ethanol, butanol can be directly injected into the car engine for use without refitting the car, will not cause rust on the engine, and will not reduce the power of the car. And butanol can use all kinds of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/225
Inventor 左文朴裴建新庞浩黄日波黄志民黎贞崇
Owner GUANGXI ACAD OF SCI