Method for preparing 5-hydroxymethyl furfural from glucose by coupling and catalyzing with isomerase/diluted hydrochloric acid
A technology for hydroxymethyl furfural and glucose, which is applied in the field of isomerase/dilute hydrochloric acid coupled catalysis to prepare 5-hydroxymethyl furfural from glucose, can solve problems such as difficult industrial application, achieve wide application, reduce operating costs, and be simple to operate Effect
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Embodiment 1
[0016] Weigh 100 g of anhydrous glucose and prepare a 50 mg / mL solution, add 105 g of sodium tetraborate (NaB 2 o 7 10H 2 O) and 5g magnesium sulfate (MgSO 4 ·7H 2 O) placed in a constant temperature water bath with a temperature of 70° C. and a rotation speed of 150 rpm. After the temperature was stabilized, 50 g of immobilized glucose isomerase was added to react for 8 hours. After the reaction, 49.5 g of immobilized glucose isomerase was recovered by suction filtration. The resulting filtrate containing glucose and fructose was analyzed and detected by high-performance liquid chromatography (HPLC), and the glucose isomerization rate reached 87.8%.
[0017] Collect 2000 mL of filtrate for acid dehydration reaction, add 45 mL of hydrochloric acid with a mass concentration of 36%, 100 g of sodium chloride and 3000 mL of n-butanol, and react in an oil bath at 190 °C for 50 min. After the reaction, the resulting solution containing 5-hydroxymethylfurfural was cooled to room...
Embodiment 2
[0019] Weigh 100 g of anhydrous glucose to prepare a 0.5 g / mL solution, add 105 g of sodium tetraborate (NaB 2 o 7 10H 2 O) and 0.5g magnesium sulfate (MgSO 4 ·7H 2 O) placed in a constant temperature water bath with a temperature of 70° C. and a rotation speed of 150 rpm. After the temperature stabilized, 50 g of immobilized glucose isomerase was added to react for 12 hours, and the immobilized glucose isomerase was recovered by suction filtration after the reaction. The resulting filtrate containing glucose and fructose was analyzed and detected by high-performance liquid chromatography (HPLC), and the glucose isomerization rate reached 86.4%.
[0020] Collect 200mL of filtrate for acid dehydration reaction, add 8.5mL of hydrochloric acid with a mass concentration of 36%, 10g of sodium chloride and 300mL of n-butanol, and react in an oil bath at 190°C for 40min. After the reaction, the resulting solution containing 5-hydroxymethylfurfural was cooled to room temperature ...
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