Cellulose decomposition compound bacteria system and method for carrying out co-culture on cellulose decomposition compound bacteria system and sulfate reducing bacteria
A cellulose decomposition and co-cultivation technology, applied in the field of microorganisms, can solve the problems of difficult decomposition, complex structure, and underutilized resources.
Inactive Publication Date: 2012-03-07
有研资源环境技术研究院(北京)有限公司
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Problems solved by technology
Due to its complex structure and difficult to decompose, most of these resources have not been fully utilized at present
Method used
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Embodiment 1
[0025] First prepare the culture medium (Tyrptone, 3g; CaCl 2 ·H 2 O, 2g; NaHCO 3 , 1g; NH 4 Cl, 1 g; distilled water, 1 L; pH 7.2). The medium was dispensed into anaerobic screw cap test tubes fitted with filter paper strips (12×120 mm). After vacuum pumping and oxygen removal, high pressure steam sterilization (121°C). Put 0.5g of anaerobic activated sludge into the sterilized culture medium, and put it into a 35°C incubator for static cultivation. After the filter paper was decomposed, the cells were successively transferred and cultured under the same culture conditions. During the transfer process, the bacterial strain's ability to decompose the filter paper was continuously enhanced, and finally the filter paper strip could be completely degraded within 48 hours. Preserve the enriched and isolated cellulolytic strains (the name of the strain is Clostridium sp.Lwy-2, deposit unit: China National Center for Typical Culture Collection, address in Wuhan University, dep...
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The invention provides anaerobic cellulose decomposition bacteria (Clostridium sp.Lwy-2, wherein the preservation register number is CCTCC NO:M208161; the preservation date is on October 17th in 2008; the preservation unit is China Center for Type Culture Collection; and the address of China Center for Type Culture Collection is in the Wuhan University.). The bacteria have strong decomposition ability for the filter paper and also show stronger decomposition ability for the straw. And the invention also provides a culture medium for carrying out enrichment and separation culture on the anaerobic cellulose decomposition bacteria, and a method for carrying out co-culture on the cellulose decomposition bacteria and sulfate reducing bacteria (Desulfovibrio halophilus RETECH-SRB-I, wherein the preservation unit is China Center for Type Culture Collection; the address is in the Wuhan University; the preservation data is on May 11th in 2007; and the preservation register number is CCTCC NO:M207060.). The method can carry out co-culture on the cellulose decomposition bacteria and the sulfate reducing bacteria and utilizes degradation products of the cellulose decomposition bacteria system for the filter paper as a carbon source of the growth of the sulfate reducing bacteria.
Description
technical field [0001] The invention relates to the technical field of microorganisms. In particular, it relates to an anaerobic bacterial composite strain with the ability to decompose cellulose and its culture method and the co-cultivation of the strain with sulfate-reducing bacteria (SRB namely: Sulfate-reducing Bcteria), to provide the carbon source required for the growth of SRB method. Background technique [0002] Using sulfate-reducing bacteria to treat acid mine wastewater is to reduce sulfate in acid mine wastewater to H 2 S, under different pH conditions, can use H 2 S selectively precipitates different heavy metals to form metal sulfides to remove heavy metal ions in wastewater and recover valuable metals. The alkalinity generated in the bioreduction reaction can neutralize acidic mine wastewater. The method has the characteristics of low cost, strong applicability, no secondary pollution, and the ability to recover heavy metals in acidic mine wastewater. How...
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IPC IPC(8): C12N1/20C12R1/01C12R1/145
Inventor 刘文彦刘兴宇陈勃伟杨丽梅
Owner 有研资源环境技术研究院(北京)有限公司