Method of detecting oleanolic acid in depression resolving pill
A technology of oleanolic acid and solution, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of unstable product quality and curative effect, inability to control quality, lack of inspection item indicators, etc., and achieve strong specificity and easy popularization. Effect
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Embodiment 1
[0012] In this example, Jieyu pills were ground into fine powder, 8g of powder was taken, and 28ml of petroleum ether at 60°C was added, soaked for 8 minutes, then filtered to remove the petroleum ether, and the remaining medicinal residues were dried, then added with 15ml of ether, soaked for 50 minutes, filtered Then the solution was concentrated to 1ml as the test solution. The above-mentioned techniques for concentrating the solution are well known to those of ordinary skill in the art. Take oleanolic acid standard substance, add ethanol to dissolve it, and concentrate to make a standard solution containing 1mg oleanolic acid per 1ml solution.
[0013] Take 5ul each of the test solution and the standard solution, drop them on the same silica gel thin-layer plate respectively, use the upper layer solution of toluene, ethyl acetate and glacial acetic acid with a volume ratio of 12:3:0.3 as the developing agent, take it out, and dry it in the air , sprayed with 8% sulfuric a...
Embodiment 2
[0015] In this example, Jieyu pills were ground into fine powder, 9g of powder was taken, added 29ml of petroleum ether at 70°C, soaked for 9 minutes, and then ultrasonically treated for 15 minutes, so that the powder of Jieyu pills and petroleum ether were fully mixed. The above ultrasonic treatment refers to ultrasonic treatment at a power of 200W and a frequency of 28KHZ, then filter to remove petroleum ether, add 16ml of ether to the remaining medicinal residue after drying, then soak for 55 minutes, filter and concentrate the solution to 1.2ml as a test solution. The above-mentioned techniques for concentrating the solution are well known to those of ordinary skill in the art. Take oleanolic acid standard substance, add ethanol to dissolve it, and concentrate to make a standard solution containing 1mg oleanolic acid per 1ml solution.
[0016] Take 5ul each of the test solution and the standard solution, drop them on the same silica gel thin-layer plate respectively, use ...
Embodiment 3
[0019] In this example, the Jieyu pills were ground into fine powder, 10 g of the powder was taken, added with 30 ml of petroleum ether at 80° C., soaked for 10 minutes, and then subjected to ultrasonic treatment for 20 minutes, so that the powder of Jieyu pills and petroleum ether were fully mixed. After ultrasonic treatment, remove petroleum ether by filtration, add ether 20ml to the remaining medicinal residues after drying, then soak for 60 minutes, and perform ultrasonic treatment for 15 minutes again, so that medicinal residues and ether are fully fused, then filter and concentrate the solution to 1.4 ml as the test solution. The above-mentioned ultrasonic treatment refers to ultrasonic treatment at a power of 200W and a frequency of 28KHZ, and the above-mentioned technique for concentrating the solution is well known to those skilled in the art. Take oleanolic acid standard substance, add ethanol to dissolve it, and concentrate to make a standard solution containing 1mg...
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