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Biological preparation for prevention and treatment of peanut root-knot nematode, and preparation method and applications thereof

A technology for biological preparations and nematodes, applied in the fields of botanical equipment and methods, biocides, nematicides, etc., can solve the problems of no root-knot nematode technology, and achieve advanced production technology, promotion of proliferation, and cost of raw materials. low effect

Inactive Publication Date: 2010-08-04
PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are also related patents on the prevention and treatment of nematodes by bacterial preparations, such as the national invention patent with the title of invention "A Bacillus licheniformis strain (B-0A12) and its preparation" and the patent No. ZL200310115535.5, which mainly reports the use of The production method of bacterial liquid inoculum for soybean cyst nematode and root rot caused by Fusarium, there is no report on the technology of killing root-knot nematode and the process technology of solid powder

Method used

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  • Biological preparation for prevention and treatment of peanut root-knot nematode, and preparation method and applications thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Strain cultivation: After preparing Gao’s No. 1 medium, fill each 500ml Erlenmeyer flask with 100ml of liquid, after sterilization, insert the strain Streptomyces sp. Fermentation culture in a shaker at a temperature of 28°C, a rotation speed of 200 rpm, and a culture time of 16 hours to obtain activated strains;

[0025] (2) Large-scale propagation of thalline: apply 1000L biological fermenter fermentation to carry out fermentation, fermented liquid is Gao Shi No. 1 substratum, and liquid filling capacity is 700L, the bacterial classification 70L of the activation that inoculation step (1) obtains, bubble enemy amount 0.05%, the initial pH was 7.2, the fermentation temperature was 30°C, and the fermentation was cultivated for 36 hours. During the period, samples were taken to check the number of bacteria under the microscope, and the bacteria had not aged yet;

[0026] (3) Bacterial agent processing: centrifuge the fermentation broth at room temperature and 6000rpm...

Embodiment 2

[0028] (1) Strain cultivation: After preparing Gaoshi No. 1 medium, fill 100ml of liquid in each 500ml Erlenmeyer flask, after sterilization, insert the strain Streptomyces sp. ℃, the rotation speed is 190 rpm, and the incubation time is 18 hours;

[0029] (2) Large-scale propagation of thalline: apply 1000L biological fermenter fermentation to carry out fermentation, fermented liquid is Gao's No. 1 substratum, and liquid filling volume is 700L, the bacterial classification 63L of the activation that inoculation step (1) obtains, bubble enemy amount 0.05%, the initial pH was 7, the fermentation temperature was 28°C, and the fermentation was cultivated for 30 hours. During the period, samples were taken to check the number of bacteria under the microscope, and the bacteria had not aged yet;

[0030] (3) Bacterial agent processing: the fermented liquid is centrifuged at room temperature and 6000rpm, and the obtained thallus and solid powder with a particle size of less than 1 mm...

Embodiment 3

[0032] The experimental determination of the effect of the biological preparation of the bacterium that embodiment 2 prevents and controls peanut root-knot nematode:

[0033] (1) test bacterial agent: the thalli that embodiment 2 obtains, thalline content is 3 * 10 8 CFU / g.

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Abstract

The invention discloses a biological preparation for the prevention and treatment of peanut root-knot nematode, and a preparation method and applications thereof. The preparation method of the biological preparation comprises the steps of: inoculating streptomyces GDPPRA NH8 in a Gause No.1 culture medium and conducting shaking cultivation to result in activated strain; then fermenting and culturing the activated strain in the Gause No.1 culture medium, wherein the loading amount is 70% of the volume of a fermentation tank, the inoculation amount is 5-10% of the volume of a fermentation culture medium, the amount of defoamer is 0.05-0.1%, the initial pH is 7-7.2, the fermentation temperature is 28-30 DEG C and the fermentation time is from 12 to 36 hours; centrifuging the fermentation liquid, mixing the resultant bacterium with solid powder with the particle size less than 1 millimeter, drying the mixture to a state that the relative humidity of water is less than 10%, and crushing the dried mixture to result in the biological preparation for the prevention and treatment of peanut root-knot nematode. The preparation method effectively promotes the proliferation of streptomyces, and the prepared biological preparation for the prevention and treatment of peanut root-knot nematode has the effect of preventing and treating peanut-parasitic root-knot nematodes as high as 85%, and the cost is low.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and in particular relates to a biological preparation for preventing and controlling peanut root-knot nematode and its preparation method and application. Background technique [0002] Plant root-knot nematodes can harm a variety of crops and cause serious damage. At present, its control method is mainly chemical control, but because chemical methods often use highly toxic and high-residue chemical pesticides, which are extremely harmful to humans and animals, some nematocides have been banned. Therefore, the development of bio-nematicides is of great significance. [0003] Among the microbial natural enemies of nematodes, there are fungi and bacteria. Among them, fungal insecticides have related patents (the name of the invention is a production method of biological nematicides, and the patent number is ZL03117930.4) and 2 commercially produced products (Royal300 produced in Fran...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01P5/00
Inventor 林壁润蒲小明沈会芳潘群英
Owner PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI