Separation method of D-glucuronic acid gamma-lactone
A glucuronic acid and separation method technology, applied in the field of separation of D-glucuronic acid γ-lactone, can solve the problems of difficult separation of crystals, easy decomposition of D-glucuronic acid, high cost, etc., to overcome the difficulty of crystal separation, The effect of reducing production cost and simplifying production process
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Embodiment 1
[0015] Example 1: 1) First, 100g corn starch, 150mL water and 0.1g FeSO 4 Add to a three-necked flask with stirring, adjust the pH to 10 with 0.1mol / L NaOH, and slowly add 6g of H while stirring 2 O 2 , React at 45℃ for 4h, after the reaction is heated to boiling to obtain oxidized starch liquid; the purpose is to allow excess H 2 O 2 Decompose by heating to avoid further oxidation of the aldehyde groups (glucuronic acid, glucose) in the enzymatic hydrolysis solution to obtain oxidized starch with carboxyl content ≥ 8% and carbonyl content ≤ 0.2%;
[0016] 2) Cool and adjust the pH of the oxidized starch solution to 6.2, add the α-amylase with an activity of 20000μ / g at the ratio of 120μα-amylase / g starch, hydrolyze it at 90℃ for 0.5h, and then heat it to boiling. Keep it for 10 minutes to kill α-amylase to obtain liquefied liquid;
[0017] 3) Cool the liquefied liquid to 62°C and adjust its pH to 4.5. Add the glucoamylase with an activity of 10000μ / g at the ratio of 120μ glucoamyla...
Embodiment 2
[0021] Example 2: 1) First, 100g corn starch, 180mL water and 0.08g FeSO 4 Add to a three-necked flask with stirring, adjust the pH to 9 with 0.1mol / L NaOH, slowly add 8g of H while stirring 2 O 2 , React at 45℃ for 4h, after the reaction is heated to boiling to obtain oxidized starch liquid; the purpose is to allow excess H 2 O 2 Decompose by heating to avoid further oxidation of the aldehyde groups (glucuronic acid, glucose) in the enzymatic hydrolysis solution to obtain oxidized starch with carboxyl content ≥ 8% and carbonyl content ≤ 0.2%;
[0022] 2) Cool and adjust the pH of the oxidized starch solution to 6.5, add the α-amylase with an activity of 20000μ / g at the ratio of 140μα-amylase / g starch, hydrolyze it at 86°C for 1.5h, and then heat it to boiling. Keep it for 10 minutes to kill α-amylase to obtain liquefied liquid;
[0023] 3) Cool the liquefied liquid to 65°C and adjust its pH to 4.2, add glucoamylase with an activity of 10000μ / g at the ratio of 140μ glucoamylase / g st...
Embodiment 3
[0027] Example 3: 1) First, 100g corn starch, 160mL water and 0.05g FeSO 4 Add to a three-necked flask with stirring, adjust the pH to 11 with 0.1mol / L NaOH, and slowly add 5g of H while stirring 2 O 2 , React at 45℃ for 4h, after the reaction is heated to boiling to obtain oxidized starch liquid; the purpose is to allow excess H 2 O 2 Decompose by heating to avoid further oxidation of the aldehyde groups (glucuronic acid, glucose) in the enzymatic hydrolysis solution to obtain oxidized starch with carboxyl content ≥ 8% and carbonyl content ≤ 0.2%;
[0028] 2) Cool and adjust the pH of the oxidized starch solution to 6.0, add the α-amylase with an activity of 20000μ / g at the ratio of 100μ α-amylase / g starch, hydrolyze at 88°C for 1h, then heat to boiling and keep 10min, kill α-amylase to obtain liquefied liquid;
[0029] 3) Cool the liquefied liquid to 61°C and adjust its pH to 4.0, add glucoamylase with an activity of 10000μ / g at the ratio of 100μ glucoamylase / g starch, and keep it...
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