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Methods of Improving the Stem Pulling Rate of Oriental Lily Test Tube Bulbs in the Year

A bulb and test tube technology, which is applied in the field of improving the rate of stem extraction of test tube bulbs of Oriental lily in the current year, can solve the problems of low stem extraction rate and thin growth, and achieves the effects of reducing workload, promoting rapid expansion and reducing cultivation costs.

Inactive Publication Date: 2011-12-21
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in practice, after planting the oriental lily test tube bulbs, the rate of stem-pulling in that year is very low, generally below 5%. This kind of bulbs without stems only grow 2 to 3 leaves on the top of the year, and the growth is relatively thin. The circumference of its underground bulbs can only grow to 4 to 6 centimeters, and it takes 4 to 5 years for the bulbs of this plant to reach the qualified standard of more than 14 centimeters.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: (improve the method 1 of oriental lily test tube bulb extraction rate in that year)

[0033] The specific method is as follows:

[0034] (1) The preparation of the culture medium: including basic medium and the components of the culture medium in each period of tissue culture, and the weight of each component per liter is:

[0035] a) basic medium: wherein,

[0036] Induction medium is sucrose 40g / L, agar powder 4g / L, MS medium with pH5.8;

[0037] Proliferation medium is 50g / L sucrose, 5g / L agar powder, 3 / 5MS medium with pH5.6;

[0038] Bulb expansion medium is sucrose 60g / L, agar powder 5.0g / L, 1 / 2MS basic medium of pH5.6;

[0039] b) Induction medium I: MS+6-BA 1.0mg / L+IBA 0.1mg / L+AC 2.0mg / L;

[0040] c) Induction medium II: MS+6-BA 1.0mg / L+IBA0.25mg / L;

[0041] d) Proliferation medium: 3 / 5MS+6-BA1.0mg / L+IBA0.5mg / L;

[0042] e) Bulb expansion medium: 1 / 2MS+NAA 0.1mg / L+PP333 1mg / L+mannitol 1.0g / L+AC 1.0mg / L;

[0043] (2) Provenance treatment and ste...

Embodiment 2

[0050] Embodiment 2: (improve the method 2 of oriental lily test tube bulb extraction rate in that year)

[0051] In this example, the preparation of step (1) medium is: a) basic medium: wherein, induction medium is sucrose 30g / L, agar powder 4.5g / L, MS medium of pH5.6; Proliferation medium is Sucrose 55g / L, agar powder 4.5g / L, 3 / 5MS medium with pH5.7; bulb expansion medium is sucrose 68g / L, agar powder 4.5g / L, 1 / 2MS basic medium with pH5.8; b) Induction medium I: MS+6-BA 2.0mg / L+IBA 0.5mg / L+AC 1.0mg / L; c) Induction medium II: MS+6-BA 0.75mg / L+IBA0.1mg / L L; d) proliferation medium: 3 / 5MS+6-BA0.2mg / L+IBA0.1mg / L; e) bulb expansion medium: 1 / 2MS+NAA 0.5mg / L+PP333 5mg / L+mannitol 5g / L+AC 5mg / L;

[0052] Step (2) Place the provenance bulbs in a 50°C incubator for heat treatment for 80min; the sterilization of the provenance bulb buds is as follows: soak in 75% alcohol for 1.0min, sterilize with 0.1% mercuric chloride solution for 20min, and sterilize with 10% sodium hypochlorite ...

Embodiment 3

[0057] Embodiment 3: (improve the method 3 of oriental lily test tube bulb extraction rate in that year)

[0058] In this example, the preparation of step (1) medium is: a) basic medium: wherein, induction medium is sucrose 50g / L, agar powder 5.0g / L, MS medium of pH5.7; Proliferation medium is Sucrose 60g / L, agar powder 4.0g / L, 3 / 5MS medium with pH5.8; bulb expansion medium is sucrose 75g / L, agar powder 4.0g / L, 1 / 2MS basic medium with pH6.0; b) Induction medium I: MS+6-BA 1.5mg / L+IBA 0.3mg / L+AC 3.0mg / L; c) Induction medium II: MS+6-BA 0.5mg / L+IBA0.5mg / L L; d) proliferation medium: 3 / 5MS+6-BA0.5mg / L+IBA0.3mg / L; e) bulb expansion medium: 1 / 2MS+NAA 0.3mg / L+PP333 10mg / L+mannitol 3g / L+AC 3mg / L;

[0059] Step (2) heat-treat the provenance bulbs in a 50°C incubator for 90min; the sterilization of the provenance bulb buds is as follows: soak in 75% alcohol for 0.75min, sterilize with 0.1% mercuric chloride solution for 18min, and sterilize with 10% sodium hypochlorite aqueous solut...

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Abstract

The invention discloses a method for increasing the stem-pulling rate of oriental lily test tube bulbs in the same year, and belongs to the technical field of plant tissue culture and rapid propagation. The method comprises: preparation of culture medium, treatment and sterilization of provenance, induction culture of bulb buds, proliferation culture of bulb buds, expansion culture of test tube bulbs, cold storage of test tube bulbs, colonization and cultivation management of test tube bulbs, and the like. The test tube bulbs cultivated by the present invention have fast seedling emergence and vigorous growth, with a diameter of 0.8 to 1.2 cm, 10 to 20 root systems per grain, a fresh weight of 0.8 to 1.2 g per grain, and a planting survival rate of over 95%. The conventional rate of less than 5% is increased to more than 60%, which accelerates the growth and expansion of bulbs and shortens the period of cultivating qualified bulbs by 1 to 2 years. The invention can be popularized and applied in oriental lily bulb production enterprises.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for increasing the rate of the oriental lily test tube bulbs in the same year. Background technique [0002] Oriental lily is a perennial ornamental plant, mainly used for cut flower cultivation. In recent years, the annual growth rate of oriental lily cut flower production in my country has reached more than 20%. The oriental lily has beautiful shape, huge flowers, bright colors, and long vase life, and is deeply loved by the people of our country. Taking advantage of climate differences at different latitudes and altitudes in my country, combined with measures such as temperature and light control of facilities, annual production can be achieved. Due to the high production efficiency and rapid development of lily cut flowers, it has become one of the important cut flower varieties. However, the oriental lily varieties currently used in cut flower producti...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 郭方其丁渭文孙崇波向林黎侠柴金甫林森洪袁峰丁晓瑜贾燕娇
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES