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Gene chip for detecting screening-gene-Hpt-containing transgenic plant and product and application thereof

A technology for genetically modified plants and screening genes, which is used in the determination/inspection of microorganisms, biochemical equipment and methods, fluorescence/phosphorescence, etc. It can solve the problems of patent reports on gene chip detection technology that have not yet discovered the Hpt gene, and achieve good specificity. Sensitivity, high sensitivity, and the effect of improving the level of supervision

Inactive Publication Date: 2012-10-03
山东鲁保科技开发有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] After searching the existing patents and other documents, no patent reports on the gene chip detection technology of Hpt gene have been found

Method used

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  • Gene chip for detecting screening-gene-Hpt-containing transgenic plant and product and application thereof
  • Gene chip for detecting screening-gene-Hpt-containing transgenic plant and product and application thereof
  • Gene chip for detecting screening-gene-Hpt-containing transgenic plant and product and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Primer and probe design

[0040] According to the Hpt gene sequence, the Primer Premier 5.0 primer design software was used to design the Hpt-specific detection primers for the screening gene, and the primers were screened and optimized. The fluorescently labeled primers are fluorescently labeled with Cy3-Amidite at the 5' end. The primers were synthesized by Shanghai Boya Biological Company, and the primers were diluted to 10 μmol / L for use.

[0041] Use Primer premier 5.0 and Oligo 6.0 software to design 40mer Oligo candidate probes based on the sequence of Hpt. The general principles of design are: (1) have relatively similar TM values, and the fluctuation range is 5°C; (2) GC content is 45% ~55%, otherwise the background of non-specific hybridization will be increased; (3) the repeated single bases shall not exceed 4 consecutively; (4) the length of the paired bases of the most stable secondary structure of the probe molecule shall not be greater than 4bp. The ...

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Abstract

The invention discloses a gene chip for detecting screening-gene-Hpt-containing transgenic plant and product and the application thereof, belonging to the field of biological engineering technology. The chip comprises a slide glass modified by surface aldehyde group, a screening-gene-Hpt probe coated with array, as well as positive control, hybridization positive control, a positive quality control probe, a negative quality control probe and blank control which are fixed by nucleic acid, wherein screening gene Hpt has the sequence of NH2-(CH2) 6-O-Poly (dT) 15-ATTTCGATGATGCAGCTGGGCGCAGGGTCGATGCGACGC. The gene chip for detecting the screening gene Hpt of the transgenic plant and the product can be used for detecting the specificity of the screening gene Hpt of the transgenic plant and the product thereof, and has higher specificity.

Description

technical field [0001] The invention relates to a gene chip for detecting transgenic plants and products containing screening gene Hpt and its application, belonging to the technical field of biochips. Background technique [0002] In recent years, genetically modified crops such as corn, soybean, cotton, rapeseed and tomato have been approved for planting and production in many countries, and some have been processed into food, feed or used as food additives. Since the ecological safety and food safety of genetically modified products have been controversial, more than 40 countries and regions have successively implemented genetically modified product labeling systems. [0003] The establishment of genetically modified labeling systems in various countries has put forward strict requirements on the sensitivity and accuracy of genetically modified detection technology, and various genetically modified detection technologies have also become research hotspots. Commonly used ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68G01N21/64
Inventor 庄乾营路兴波刘同金王鹏
Owner 山东鲁保科技开发有限公司