Crystal of rat RANK/RANKL extracellular compound, preparation method and application thereof

A complex and crystal technology, which is applied in the field of purifying and expressing RANK extracellular protein and preparing mouse RANK/RANKL extracellular complex crystals, which can solve the problems of difficult to obtain crystals and large variability.

Inactive Publication Date: 2010-09-29
GENERAL HOSPITAL OF PLA +1
View PDF1 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

2. Compared with the relatively rigid structure of TNF protein family members including RANKL, the structure of TNFR protein family members including RANK has greater variability, so it is more difficult to obtain crystallization

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Crystal of rat RANK/RANKL extracellular compound, preparation method and application thereof
  • Crystal of rat RANK/RANKL extracellular compound, preparation method and application thereof
  • Crystal of rat RANK/RANKL extracellular compound, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0031] 1. Expression and purification

[0032] 1. Expression and purification of mouse RANK extracellular domain protein

[0033] The amino acid sequence of mouse RANK is as follows:

[0034] MAPRARRRRQLPAPLLALCVLLVPL QVTLQVTPPCTQERHYEHLGRCCSR CEPGKYLSSKCTPTSDSVCLPCGPDEYLDTWNEEDKCLLHKVCDAGKALV AVDPGNHTAPRRCACTAGYHWNSDCECCRRNTECAPGFGAQHPLQLNKDT VCTPCLLGFFSDVFSSTDKCKPWTNCTLLGKLEAHQGTTESDVVCSSSMT LRRPPKEAQA YLPSLIVLLLFISVVVVAAIIFGVYYRKGGKALTANLWNWVNDACSSLSGNKESSGDRCAGSHSATSSQQEVCEGILLMTREEKMVPEDGAGVCGPVCAAGGPWAEVRDSRTFTLVSEVETQGDLSRKIPTEDEYTDRPSQPSTGSLLLIQQGSKSIPPFQEPLEVGENDSLSQCFTGTESTVDSEGCDFTEPPSRTDSMPVSPEKHLTKEIEGDSCLPWVVSSNSTDGYTGSGNTPGEDHEPFPGSLKCGPLPQCAYSMGFPSEAAASMAEAGVRPQDRADERGASGSGSSPSDQPPASGNVTGNSNSTFISSGQVMNFKGDIIVVYVSQTSQEGPGSAEPESEPVGRPVQEETLAHRDSFAGTAPRFPDVCATGAGLQEQGAPRQKDGTSRPVQEQGGAQTSLHTQGSGQCAE

[0035] The underlined part is the amino acid sequence of the extracellular region of mouse RANK

[0036] Material

[0037] Oligonucleotides were prepared...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for purifying and expressing RANK extracellular area protein, and also provides a method for preparing RANK/RANKL compound crystals, a three-dimensional crystal structure of the RANK/RANKL compound, and applications of the crystal in the medical field.

Description

technical field [0001] The invention relates to a method for purifying and expressing RANK extracellular protein, and also provides a crystal of RANK / RANKL complex, especially a crystal formed of mouse RANK / RANKL extracellular protein. The invention provides a method for preparing rat RANK / RANKL extracellular complex crystal and the application of the complex structure in the field of medicine. Background technique [0002] Cytokines in the tumor necrosis factor (TNF) family play important roles in various biological functions such as inflammatory response, organogenesis, host defense, autoimmunity and apoptosis. These biological regulatory factors play corresponding physiological roles by triggering intracellular signal transmission and changing cell phenotype through receptor-ligand interaction. As a member of the TNF family, RANKL and its receptor RANK play an important role in various activities in the body, and play an important role in regulating the bone remodeling p...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00C12N15/70C12N15/74C12P21/02C30B29/54G01N33/68C12R1/19
Inventor 唐佩福刘长振黄鹏张世谦高斌
Owner GENERAL HOSPITAL OF PLA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap