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Cell composition for tissue regeneration

A composition and tissue technology, applied in the field of human cell population, can solve problems such as expensive and time-consuming

Inactive Publication Date: 2010-09-29
REGENETECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in various cases it is considered important to ensure that any traces of cord blood are eliminated, an expensive and time-consuming procedure

Method used

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  • Cell composition for tissue regeneration
  • Cell composition for tissue regeneration
  • Cell composition for tissue regeneration

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Experimental program
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Embodiment

[0062] Harvesting progenitor cells from human umbilical jelly

[0063] Umbilical cords were collected from full-term cesarean infants immediately after delivery. The umbilical cord was then transferred by the surgeon into a sterile container containing medium (80% α-MEM, 20% antibiotics).

[0064] From this point forward, all steps are performed aseptically in a biosafety cabinet. Umbilical cord in phosphate-buffered saline (PBS) (--Mg 2+ , --Ca 2+ ) to remove as much cord blood as possible, and transferred back to the container containing the culture medium. An approximately 6 cm length of the umbilical cord was cut with sterile scissors and placed on a sterile cork dissecting board. The rest of the umbilical cord (30-45 cm) was returned to the medium-filled container and placed in an incubator at 37°C. A 6 cm segment of umbilical cord was "twisted" about its helix and stapled at both ends to expose the smooth and straightened surface of the umbilical cord epithelial t...

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Abstract

A method of extracting human progenitor cells from perivascular tissue of human umbilical cord. The extracted cells are then co-cultured with hemotopoetic stem cells and are useful to grow and repair human tissues including bone. Also included are related methods and compositions related thereto.

Description

field of invention [0001] The present invention focuses on harvesting rapidly proliferating human cell populations from the connective tissues of the umbilical cord, methods for co-cultivating these cells with hematopoietic stem cells, compositions related thereto, and compositions useful for various cell types of therapy. Background technique [0002] Obtaining therapeutic cell mixtures from umbilical cord jelly is well known. However, in various cases it is considered important to ensure that any trace of cord blood is eliminated, an expensive and time-consuming procedure. The present invention does not suffer from this problem. In the present invention, cells derived from umbilical cord jelly are co-cultured with hematopoietic stem cells. [0003] The umbilical cord is one of the first structures that forms what is later gastrulation (the formation of the three germ layers). As it begins to fold, the blastodisc begins to connect via the protomidgut (embryonic origin) t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/14A61K35/28A61K35/50C12N5/06A61K35/51C12N5/0775
CPCC12N5/0668A61K35/51C12N2502/1171C12N5/0605A61K35/28A61K2300/00
Inventor 弗朗西丝卡·P·维特利戴维·A·沃尔夫唐尼·拉德
Owner REGENETECH INC