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Tissue culture method of short pampas grass

A technology of tissue culture and dwarf pampas grass, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of small number of dwarf pampas grass introduced and limited supply of seedlings, etc., so as to improve the propagation speed and the neatness of seedlings degree, the effect of improving stability

Inactive Publication Date: 2012-01-04
SHANGHAI SHANGFANG LANDSCAPE PLANT INST +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as a new variety introduced from abroad, the number of introduced species of pampas grass is relatively small, and the supply of seedlings is limited to a certain extent.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Obtaining sterile materials

[0029] Take the germinated young branches in spring, remove the branches and leaves, wash them with tap water for 1 hour, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 70% for 10 seconds, and mercury with a volume concentration of 0.5‰ for 10 minutes, and then use sterile Rinse with water for 4 times, dry the water on the surface with sterile filter paper, then cut into 0.5cm-long segments with axillary buds, and inoculate the segments with MS+6-BA1.0mg / L+NAA0.1mg / L on axillary bud induction medium;

[0030] (2) Differentiation and proliferation of buds

[0031] After the segments were inoculated on the axillary bud induction medium for 1 week, the axillary buds began to expand and green protrusions appeared. After 3 weeks, bud meristems could be seen. After culturing for 1 month, the small adventitious buds could grow to 3 cm. Cut the adventitious buds Under transfer into the adventitious bu...

Embodiment 2

[0040] (1) Obtaining sterile materials

[0041] Take the germinated young branches in spring, remove the branches and leaves, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 75% for 30 seconds, and soak them in mercury with a volume concentration of 1‰ for 15 minutes, and then use sterile Rinse with water 5 times, use sterile filter paper to blot the surface moisture, then cut into 1cm-long segments with axillary buds, and inoculate the segments with axillary buds containing MS+6-BA5.0mg / L+NAA0.5mg / L on the induction medium;

[0042] (2) Differentiation and proliferation of buds

[0043] After the segments were inoculated on the axillary bud induction medium for 2 weeks, the axillary buds began to expand and green protrusions appeared. After 4 weeks, bud meristems could be seen. After one and a half months of culture, the small adventitious buds could grow to 4cm. Cut the adventitious buds Under t...

Embodiment 3

[0052] (1) Obtaining sterile materials

[0053] Take the germinated young branches in spring, remove the branches and leaves, wash them with tap water for 3 hours, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 75% for 50 seconds, and then soak them in mercury with a volume concentration of 2‰ for 30 minutes. Rinse with water for 6 times, use sterile filter paper to blot the water on the surface, then cut into 2cm-long segments with axillary buds, and inoculate the segments with axillary buds including MS+6-BA5.0mg / L+NAA0.5mg / L on the induction medium;

[0054] (2) Differentiation and proliferation of buds

[0055] After the segments were inoculated on the axillary bud induction medium for 3 weeks, the axillary buds began to expand and green protrusions appeared. After 5 weeks, bud meristems could be seen. After 2 months of culture, the small adventitious buds could grow to 4cm. Cut the adventitious buds Under transfer into the adventi...

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PUM

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Abstract

The invention relates to a tissue culture method of short pampas grass, comprising the following steps of: acquisition of a sterile material, differentiation and propagation of buds, strong seeding culture of adventitious buds, rooting culture, seedling hardening, transplanting, and the like. Compared with the prior art, the invention improves the propagation speed of the short pampas grass, the neatness of the seedlings and the stability of properties of the short pampas grass and can realize industrial large-batch production of the seedlings.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a dwarf pampas grass tissue culture method. Background technique [0002] Dwarf pampas grass is a plant of the genus Pampas grass in the grass family, widely distributed, and is an evergreen perennial herb with a plant height of 80-100cm, large panicles, feathery, silvery white, and is a famous ornamental grass abroad. Can be used for landscaping or shore. The spikes of dwarf pampas grass are long and beautiful, which is spectacular and elegant in garden cultivation, or planted on the bank, and enjoy the panicles of its silvery-white pinnate spikes in autumn, and can also be used as dried flowers, or used in flower border ornamental grass gardens , has excellent ecological adaptability and ornamental value, and is a good flower mirror and ground cover material. However, as a new variety introduced from abroad, the number of introduced species of pampas pampas grass is relatively...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 陈建华黄建荣沈勤
Owner SHANGHAI SHANGFANG LANDSCAPE PLANT INST