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Blood sample protecting solution and method for collecting blood sample

A blood sample and protective solution technology, applied in the direction of blood/immune system cells, vertebrate cells, animal cells, etc., can solve the problems of blood corruption, affecting the accuracy of test results, bacterial contamination, etc., to achieve less pollution and save reagent costs , the effect of reducing demand

Active Publication Date: 2014-04-16
樊福好
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After the blood sample is collected by the existing method, it is necessary to coagulate the blood sample in advance to separate the serum, but the blood coagulation process is a complex biochemical reaction process, which can easily lead to hemolysis, lipemia, bacterial contamination and blood corruption, seriously affecting Accuracy of test results

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] First take 10,000 units of sialidase, 20 μm of Tris (trishydroxymethylaminomethane), 3 ml of 10 μm silica particles, ddH 2 O 94 ml prepared sample protection solution.

[0015] Draw 5 ml of the prepared sample protection solution with a disposable syringe;

[0016] Draw 5 ml of blood from the pig vein with the syringe that has taken the sample protection solution, and mix the blood with the sample protection solution evenly;

[0017] Send the above-mentioned syringe containing the mixture of blood sample and sample protection solution to the laboratory, and the mixture must not be shaken or frozen;

[0018] Natural precipitation of the mixed liquid of blood sample and sample protection solution;

[0019] Take the supernatant for antibody or antigen analysis.

Embodiment 2

[0021] First take 10,000 units of sialidase, 20 μm of Tris (trishydroxymethylaminomethane), 3 ml of 10 μm silica particles, ddH 2 O 94 ml prepared sample protection solution.

[0022] Draw 5 ml of the prepared sample protection solution with a disposable syringe;

[0023] Draw 5 ml of blood from the pig vein with the syringe that has taken the sample protection solution, and mix the blood with the sample protection solution evenly;

[0024] Transfer the above-mentioned mixed liquid containing blood sample and sample protection solution to a centrifuge tube and send it to the laboratory. The mixed liquid must not be shaken or frozen;

[0025] Centrifuge the mixed liquid of blood sample and sample protection solution with a centrifuge;

[0026] Take the supernatant for antibody or antigen analysis.

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PUM

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Abstract

The invention discloses blood sample protecting solution. The sample protecting solution is prepared from 1.2 to 3 volume percent of sialidase, 0.5 to 2.5 volume percent of tromethamine, 0.8 to 2.0 volume percent of silicon dioxide granules of 10 microns and 92 to 96 volume percent of ddH2O. Meanwhile, the invention also discloses a method for collecting a blood sample, which comprises the following steps of: extracting the prepared sample protecting solution of a certain volume by using an injector, extracting the blood of the same volume, mixing the blood and the protecting solution uniformly, conveying the mixed solution together with the injector to a laboratory, or transferring the mixed solution to a centrifuge tube and then conveying the mixed solution and the centrifuge tube to the laboratory, and finally centrifuging or naturally settling the mixed solution for later use. When the blood sample collected by using the method is used, the blood sample and the protecting solution are mixed according to a certain proportion so that the blood sample is not easily polluted and corrupted, the preservation time of the blood sample is prolonged and the detection result of the blood sample is more accurate; and meanwhile, the demand of the sample can be reduced, and the cost of reagents can be saved.

Description

technical field [0001] The invention relates to a protective solution for protecting blood samples, and also relates to a method for collecting blood samples. Background technique [0002] Using the specific reaction between antigens and antibodies can help detect specific antibodies or specific antigens in the blood to determine the cause, so blood collection and analysis is one of the most common methods in the diagnosis of some diseases and diseases . After the blood sample is collected by the existing method, it is necessary to coagulate the blood sample in advance to separate the serum, but the blood coagulation process is a complex biochemical reaction process, which can easily lead to hemolysis, lipemia, bacterial contamination and blood corruption, seriously affecting Accuracy of test results. Contents of the invention [0003] The object of the present invention is to provide a kind of protective solution that avoids blood sample hemolysis, lipemia, bacterial co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/078
Inventor 樊福好
Owner 樊福好