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Kit for detecting therapeutic effects of IRESSA and TARCEVA for treating non-small cell lung cancer

A technology for non-small cell lung cancer, curative effect, applied in biochemical equipment and methods, microbial determination/inspection, material excitation analysis, etc.

Inactive Publication Date: 2010-12-29
XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current clinical studies have proven that for NSCLC with mutations in the coding region of the EGFR tyrosine kinase gene (exons 18, 19, and 21), the effective rate of Gefitinib is as high as 80%, while for wild-type EGFR-positive tumors without gene mutations, The above drug treatment is basically ineffective

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] Below in conjunction with specific embodiment, further illustrate the present invention. For the experimental methods not indicated in the following examples, the specific conditions are usually in accordance with conventional conditions, or in accordance with the conditions suggested by the manufacturer.

[0021] The use of embodiment detection kit

[0022] Step 1: Extraction of DNA template

[0023] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0024] Step 2: Real-time quantitative PCR reaction

[0025] Use the fluorescent quantitative PCR kit in the detection kit to carry out the fluorescent quantitative PCR reaction. The reaction system has a total volume of 10 μl, including 2 μl of DNA template with a concentration of 20 ng / μl, 1 μl of 10X fluorescent quantitative PCR reaction buffer, and 0.1 μl of 25 mM dNTP mixture solution, 0.6μl 25mM MgCl 2 solution, 0.025 μl (5 units / μl) Taq DNA polymerase, 0.225 μl each of 20 μM sense prim...

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Abstract

The invention disclose a kit for detecting the therapeutic effects of individual IRESSA and TARCEVA for treating a non-small cell lung cancer, comprising a specific primer pair, a specific fluorescent probe pair, a fluorescent quantitative PCR (Polymerase Chain Reaction) conventional assembly, and the like, wherein the specific primer pair and the specific fluorescent probe pair are used for detecting EGFR (Epidermal Growth Factor Receptor) gene polymorphism sites which are the medicated targeting sites of the IRESSA and the TARCEVA. The kit estimates the therapeutic effects of the individual IRESSA and TARCEVA for treating the non-small cell lung cancer by detecting the EGFR gene polymorphism sites which are the medicated targeting sites of the IRESSA and the TARCEVA.

Description

technical field [0001] The present invention relates to the fields of molecular biology and medicine. More specifically, the present invention relates to a kit for detecting the curative effect of individual IRESSA and TARCEVA on non-small cell lung cancer. Points to evaluate the efficacy of individual IRESSA and TARCEVA in the treatment of non-small cell lung cancer. Background technique [0002] Lung cancer is the first cause of cancer death in China and even in the world. Lung cancer can be divided into two categories: non-small cell lung cancer and small cell lung cancer. Among them, non-small cell lung cancer is the most common, accounting for 80% of the total number of lung cancers. There are three distinct types of NSCLC: adenocarcinoma (including bronchoalveolar cell carcinoma), squamous cell carcinoma, and large cell undifferentiated carcinoma. As far as advanced lung cancer is concerned, the current effective rate of first-line chemotherapy is only about 30%. For...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N21/64
Inventor 冯哲民邹祖烨
Owner XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI