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Honeysuckle extract preparation method

A honeysuckle extract and honeysuckle technology are applied in the extraction and purification of active components of traditional Chinese medicine, and the preparation of honeysuckle extracts, and can solve the problems of low purity of active components, long extraction time, and much solvent consumption, and achieve short extraction time and solvent consumption. Low dosage and easy operation

Active Publication Date: 2012-06-13
蚌埠丰原涂山制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But above-mentioned these traditional methods, the adding amount of its solvent is at least more than 10 times of honeysuckle, and needs extraction at least 2~5 hours, consumes many solvents and extraction time is long; And because the composition of honeysuckle medicinal material is complicated, contains many organic acids Therefore, the extracts obtained by traditional methods have low purity of active ingredients (such as organic acids)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Ultra-high pressure extraction: Take 500g of honeysuckle medicinal material, break it into small pieces of about 3mm in length, put it into an ultra-high pressure extraction tank, add 3.5L 75% ethanol; pressurize to 200MPa, keep the pressure for 15min, release the pressure immediately, filter, and the filtrate After concentrating to 30% of the original volume, refrigerate overnight; centrifuge the refrigerated liquid at a speed of 5000r / min for 20min;

[0020] 2. Purification: The supernatant obtained after centrifugation is decompressed to recover ethanol to liquid extract, then add 500mL water to dissolve, put it on a macroporous adsorption resin column (model: D-101), and use 5 times the volume of the resin column in turn Water elution, then elution with 20% ethanol of 8 times of resin column volume, decompression reclaims ethanol eluate to no alcohol smell, freeze-drying, obtains honeysuckle extract 11.41g, wherein the content of total organic acid of active ingre...

Embodiment 2

[0022] 1. Take 500g of honeysuckle medicinal material, break it into small pieces of about 5mm in length, put it into an ultra-high pressure extraction tank, and add 4.5L of 80% methanol. Pressurize to 100Mpa, keep the pressure for 30min, release the pressure immediately, filter, concentrate the filtrate to 20% of the original volume, and refrigerate overnight; then centrifuge the refrigerated solution at a speed of 5000r / min for 15min.

[0023] 2. Recover methanol from the supernatant obtained after centrifugation under reduced pressure to a liquid extract, then add 500mL of water to dissolve it, put it on a macroporous adsorption resin column (model: HP-20), and use 8 times the volume of the resin column in turn. Elution, then elution with 25% ethanol of 10 times the amount of resin column volume, reclaim the ethanol eluent under reduced pressure to no alcohol smell, freeze-drying, get honeysuckle extract 11.33g, wherein the content of active ingredient total organic acid is ...

Embodiment 3

[0025] 1. Ultra-high pressure extraction: Take 500g of honeysuckle medicinal material, break it into small pieces of about 10mm long, put it into an ultra-high pressure extraction tank, add 4.0L 90% isopropanol; pressurize to 400MPa, keep the pressure for 25min, release the pressure immediately, filter, After concentrating the filtrate to 30% of the original volume, refrigerate overnight; centrifuge the refrigerated liquid at a speed of 5000r / min for 15min;

[0026] 2. Purification: The supernatant obtained after centrifugation is decompressed to recover ethanol to liquid extract, then add 500mL water to dissolve, put it on a macroporous adsorption resin column (model: SP-70), and use 8 times the volume of the resin column in turn Eluted with water, then eluted with 30% n-butanol of 8 times the volume of the resin column, recovered the ethanol eluate under reduced pressure until it had no alcohol smell, and freeze-dried to obtain 11.51g of honeysuckle extract, wherein the activ...

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PUM

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Abstract

The invention provides a honeysuckle extract preparation method, which comprises the following: 1) a step of ultrahigh-pressure extraction which comprises adding an alkanol solvent into honeysuckle, increasing pressure to 100 to 400 MPa, keeping the pressure for 15 and 30min, decompressing, filtering, concentrating filtrate, refrigerating the concentrated product and centrifugally separating; and2) a step of purification which comprises recovering supertenant obtained by centrifuging till the supertenant becomes fluid extract first, adding water into the fluid extract to dissolve the fluid extract, and pouring the obtained solution onto a macroporous adsorbent resin column then, eluting the macroporous adsorbent resin column with water in an amount which is 5 to 10 times the volume of the resin column next, eluting with 20 to 30 percent alkanol solvent in an amount which is 6 to 10 times the volume of the resin column later, and recovering the alcohol eluent till the solvent has no alcohol smell and obtaining the honeysuckle extract by freezing and drying at last. The method of the invention has the advantages that: ultrahigh-pressure extraction and macroporous absorbent resin separation technology is used to extract and purify the active ingredients of the honeysuckle, so operation is simple and convenient, the used amount of the solvent is small, extraction time is short and cost is low; the yield of the extracted active ingredients is high, and repeatability and stability are high; and raw material sources can be fully used, and industrial production can be realized easily.

Description

technical field [0001] The invention relates to a method for extracting and purifying active components of traditional Chinese medicines, in particular to a method for preparing a honeysuckle extract. It belongs to the technical field of traditional Chinese medicine preparation. Background technique [0002] Honeysuckle is first recorded in Liang Tao Hongjing's "Bielu of Famous Doctors", and its name is Lonicera. "Chinese Pharmacopoeia" (1963 edition) regards honeysuckle as the only source of honeysuckle. After 1977, each edition of "Chinese Pharmacopoeia" comprehensively considers factors such as clinical efficacy and regional habits, and selects Lonicera japonica Thunb. Honeysuckle Lonicera hypoglauca Miq., Lonicera confusa DC., or Lonicera dasystyla Rehd. have dried flower buds or early blooming flowers of honeysuckle, and the authentic product is the dried flower buds of Lonicera, which are produced in most parts of my country. Shandong has the largest output, and Henan ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/355A61P31/04A61P31/12
Inventor 陈文明汪洪湖
Owner 蚌埠丰原涂山制药有限公司