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Method for quantification of target substance

A target substance, purpose technology, applied in the field of determination of molecules, can solve problems such as unsuitability for long-term use, protein chemistry and thermal instability life limit

Inactive Publication Date: 2014-08-13
SONY CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Unfortunately, because proteins are limited in lifetime due to their chemical and thermal instability, and they undergo nonspecific adsorption, they are not suitable for long-term use

Method used

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  • Method for quantification of target substance

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Embodiment Construction

[0035] The following is a description of preferred embodiments of the present invention given with reference to the accompanying drawings. It should be noted that the embodiments are merely typical examples, and should not be construed as limiting the scope of the present invention.

[0036] figure 1 is a schematic diagram showing a method for measuring a target substance according to the present invention. "T" represents a target substance, "A" represents an aptamer that specifically binds to the target substance, and "N" represents a nucleic acid strand having a base sequence complementary to at least a part of aptamer A.

[0037] The method according to the present invention is expected to determine the target substance T by competitively binding the aptamer A specifically binding to the target substance T with the target substance T and a nucleic acid strand N having a base sequence complementary to at least a part of the aptamer A. (see figure 1 (1)). In this state, t...

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Abstract

The present invention discloses a method for quantifying a target substance with high sensitivity by means of an aptamer. Specifically disclosed is a method for quantifying a target substance, the method comprising the following steps: under the condition that the target substance and the nucleic acid chain are competitively combined with each other, an aptamer capable of specifically binding the target substance is combined with the The nucleic acid strand of the base sequence complementary to at least a part of the aptamer binds, detects at least one of a physical change and a chemical change caused by the binding; and quantifies the target substance based on the detection result.

Description

technical field [0001] The invention relates to a method for assaying molecules. More specifically, the present invention relates to a method for determining molecules by means of aptamers. Background technique [0002] Biosensors consist of a material capable of recognizing molecules and a transducer that detects physical and / or chemical signals resulting from the interaction between this material and a target chemical. Traditional materials capable of recognizing molecules are proteins such as enzymes, antibodies and receptors. [0003] Document 1 (BUNSEKI KAGAKU Vol. 51, No. 6, (2002) 389-396) discloses a method for high-speed screening of endocrine disrupting chemicals by means of a surface plasmon resonance (SPR) sensor. This method involves immobilizing the hormone-responsive sequence DNA (ERE) of the promoter region of the estrogen-responsive gene on the SPR sensor chip, and adding purified estrogen receptor α (ER) to it, so that without modification Real-time meas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6825C12Q1/6816C12Q2545/114C12Q2537/161C12Q2525/205C12Q2565/632C12Q2565/107C12N15/115C12N2310/16C12Q2525/301G01N33/48
Inventor 原田智子渡部祐己
Owner SONY CORP
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