Method for extracting phellopterin from changium smyrnioide root bark

A kind of technology of saponin and Ming Codonopsis, which is applied in the field of purifying palindrome, can solve the problems of less research on palindrome, stagnation of polysaccharides and amino acids, and undiscovered extraction process literature and patents, etc., to achieve content stability and production The effect of low cost and high content

Inactive Publication Date: 2011-01-26
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, the research on all aspects of Codonopsis pilosula is still stagnant in the study of the structure of polysaccharides a

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0019] Example one:

[0020] Put 1kg Ming Codonopsis root bark powder into the extraction tank, add 10L methanol and heat reflux for extraction for 2 hours, filter, extract 3 times, combine the extracts, add 0.5% NaOH solution to the pH of the total solution, filter, and filter the filtrate with weak acid cation exchange resin The column is exchanged until the lowering liquid is neutral. Collect the lowering liquid, add 0.5mol / L HCl solution to adjust the pH to 5, and let it stand at -4°C until no precipitation is precipitated. Filter the precipitate and add an appropriate amount of methanol to dissolve it. The XSD-1 macroporous adsorption resin column was saturated and adsorbed for 1 hour, and eluted with ether:methanol (8:2). The elution process was detected by HPLC. When the elution reached the end, the eluent was stopped and the eluent was decompressed to recover the solvent. Drying to obtain the coralline lactone refined extract powder, adding ethyl acetate-ether (4:1) to re...

Example Embodiment

[0021] Embodiment two:

[0022] Take 1kg Ming Codonopsis root bark powder, add 15L acetone solution and mix; then put the mixture in the ultrasonic generator container, use ultrasound to ultrasonically extract Ming Codonopsis root bark powder with a power of 150W, filter to remove the drug residue, and extract 3 Next, combine the extracts, adjust the pH of the extract to 10 with 0.8% NaOH solution, filter, exchange the filtrate with a weakly acidic cation exchange resin column until the lowering liquid is neutral, collect the lowering liquid, add 0.1mol / L HCl solution to adjust the pH to 6. Let stand at -5°C until there is no precipitation, filter the precipitate, add appropriate amount of methanol to dissolve it, put it on AB-8 macroporous adsorption resin column for saturated adsorption for 1.5h, elution with chloroform: methanol (7:3), The elution process was detected by HPLC, and the eluent was stopped at the end of elution. The solvent was recovered under reduced pressure fr...

Example Embodiment

[0023] Example three:

[0024] Put 2kg Ming Codonopsis root bark powder into the extraction tank, add 35L boiling water to percolate and extract for 6 hours, extract 3 times, combine the extracts, add 0.3% NaOH solution to the total solution pH 8, filter, and exchange the filtrate on a weak acid cation exchange resin column Until the betting liquid is neutral, collect the betting liquid, add 0.2mol / L HCl solution to adjust the pH to 4, and let it stand at 0℃ until no precipitation is precipitated. Filter the precipitate, add an appropriate amount of methanol to dissolve, and add XSD- 1 The macroporous adsorption resin column is saturated and adsorbed for 1 hour, and eluted with ether:ethanol (4:6). The elution process is detected by HPLC. The eluent is stopped at the end of the elution. The eluent is decompressed to recover the solvent and dried to obtain coral The refined extract powder of brassinolide is recrystallized by adding ethyl acetate-diethyl ether (3:1) and dried to ob...

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Abstract

The invention relates to a method for extracting phellopterin from changium smyrnioide root bark. The method mainly comprises the following steps of: heating and extracting changium smyrnioide root bark powder serving as a raw material with a solvent of 5 to 20 times; adding sodium hydroxide into filtrate; filtering; introducing a weakly acidic cation exchange resin into the filtrate; collecting bottom poured solution; adding acid to adjust pH; standing at a low temperature until precipitates are not separated out; filtering the precipitates; adding an appropriate amount of methanol or ethanol for dissolving; loading on a macroporous adsorption resin column; collecting eluent; performing exsolution and drying to obtain refined extract powder; and recrystallizing with an ethyl acetate-ether mixed solvent so as to obtain the phellopterin. The method has the advantages of simple extraction and purification processes, capability of obtaining the phellopterin of which the purity is up to 90 percent and more suitability for building an environmentally-friendly and resource-saving natural product development system.

Description

Technical field: [0001] The invention relates to a method for preparing natural chemicals, and in particular to a method for purifying pectinolactone from the root bark of Codonopsis tangensis. Background technique: [0002] Background technology: phellopterin, also known as phellopterin, has a molecular formula of C17H16O5 and is a furocoumarin substance present in plants such as Angelica dahurica, Fangfeng, Mingdangshen, and Notopterygium broad-leaved plants. [0003] Ming Codonopsis Changium smyrnioides Wolff is a perennial herbaceous plant of Umbelliferae (Umbellifarae), whose peeled roots are used for medicinal purposes. It was first recorded in the Ming and Qing Dynasties, and it is a valuable traditional Chinese medicine in the current version of "Chinese Pharmacopoeia". Distributed in Jiangsu, Anhui, Zhejiang, Jiangxi, Hubei and other provinces, Jiangsu is the production area of ​​authentic medicinal materials, and it is a third-level rare and endangered plant under...

Claims

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Application Information

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IPC IPC(8): C07D493/04
Inventor 刘东锋郭琴杨成东
Owner NANJING ZELANG MEDICAL TECH
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