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Method for detecting surface difference membrane protein of mesenchymal stem cell of umbilical cord source

A technology for stem cells and membrane proteins, which is used in measurement devices, material analysis by electromagnetic means, instruments, etc., to reduce the false positive rate and false negative rate, and reduce errors.

Active Publication Date: 2013-07-31
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As the pipeline for umbilical cord blood delivery—the umbilical cord, there is currently no information indicating the difference between the surface expression proteins of MSCS isolated from umbilical cord blood and MSCS from umbilical cord blood

Method used

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Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0021] The technical solutions of the present invention will be described in detail below in conjunction with specific implementation examples.

[0022] First of all, it is stated that although the present invention involves the use of fresh umbilical cord tissue and fresh umbilical blood, it only targets fresh umbilical cord tissue and fresh umbilical blood that have been separated from the human body, and the specific acquisition process itself does not belong to the content of the present invention. The detailed description of the preparation method in the present invention is only for the purpose of more clearly introducing the preparation process of biological materials, but this description does not mean that related technical means also belong to the content claimed in the present invention.

[0023] (1) Isolation and culture of mesenchymal stem cells

[0024] Isolation and culture of umbilical cord MSCSs:

[0025] Under aseptic conditions, the umbilical cord was full...

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Abstract

The invention relates a method for detecting a difference membrane protein of a stem cell, and aims to provide a method for detecting the surface difference membrane protein of the mesenchymal stem cell of an umbilical cord source. The method comprises the following steps of: separating and cultivating the mesenchymal stem cell, extracting the membrane protein of the mesenchymal stem cell, obtaining a membrane protein gel graph by a two-directional fluorescence difference gel electrophoresis (2D-DIGE) method, and analyzing and checking the mass spectrum. In the method, the 2D-DIGE method is first used for detecting a special difference surface marker of the membrane surface of the stem cell so as to perform quantitative comparison and analysis on proteins in different samples on the same gel; an interior label is added into each gel; and DIA and BVA software can automatically calibrate an expression quantity of each protein point according to the interior label of the protein point, so the error between gel and gel in different batches is reduced to the maximum extent, the real change degree of the protein is reflected, and the false positive rate and the false negative rate are lowered.

Description

field of invention [0001] The invention relates to a method for detecting differential membrane proteins of stem cells, in particular to a method for detecting differential membrane proteins on the surface of umbilical cord and umbilical cord blood mesenchymal stem cells (Mesenchymal stem cells, MSCSs). Background technique [0002] Stem cells (stem cells, SC) are a kind of pluripotent cells with self-replication ability, which can differentiate into various functional cells under certain conditions. Mesenchymal stem cells (MSCSs) are an important member of the stem cell family, derived from the mesoderm during embryonic development, mainly present in the connective tissue and interstitium of organs, and abundant in bone marrow tissue. Because of its strong proliferation ability and multi-directional differentiation potential, hematopoietic support and promotion of stem cell implantation, immune regulation and other characteristics, it has broad clinical application prospect...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/447G01N27/64
Inventor 曹红翠李兰娟俞炯
Owner ZHEJIANG UNIV