Method for detecting surface differential membrane protein of mesenchyme stem cells of placenta source
A technology of mesenchymal stem cells and membrane proteins, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of no clear cell surface markers, avoid accidental and non-parallel, reduce false positive rate and false negative rate, reduce effect of error
Active Publication Date: 2011-06-15
ZHEJIANG UNIV
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Problems solved by technology
In other words, neither bone marrow, placenta nor umbilical cord MSCS have clear cell surface markers
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The invention relates to a method for detecting differential membrane protein of mesenchyme stem cells, and aims at providing a method for detecting surface differential membrane protein of mesenchyme stem cells of a placenta source. The method comprises the steps of: separating and culturing mesenchyme stem cells, extracting membrane protein from mesenchyme stem cells, obtaining membrane proteingel spectrum by bidirectional fluorescence difference gel electrophoresis, and carrying out mass spectrum analysis and verification. The method utilizes 2D-DIGE (Difference Gel Electrophoresis) for detecting special differential surface markers on the surface of the membrane of the stem cells for the first time, and can compare and analyze protein in different samples of the same gel quantitatively; an interior marker is added in each gel, and DIA and BVA software can automatically calibrate the expression quantity of protein according to the interior marker in each protein point, thus the error between gel of different batches is reduced to the maximum extent, the real change degree of protein is reflected, and the false positive rate and the false negative rate are reduced.
Description
Detection method of differential membrane proteins on the surface of placenta-derived mesenchymal stem cells field of invention The invention relates to a method for detecting differential membrane proteins of stem cells, in particular to a method for detecting differential membrane proteins on the surface of mesenchymal stem cells (Mesenchymalstemcells, MSCSs) derived from placenta and umbilical cord. Background technique Stem cells (stem cells, SC) are a kind of pluripotent cells with self-replication ability, which can differentiate into various functional cells under certain conditions. Mesenchymal stem cells (MSCSs) are an important member of the stem cell family, originally derived from bone marrow, because of their strong proliferation ability and multi-lineage differentiation potential, hematopoietic support and promotion of stem cell implantation, immune regulation and other characteristics make them useful in tissue engineering, Cell therapy and gene therapy have...
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Login to View More IPC IPC(8): G01N27/447G01N27/64
Inventor 李兰娟曹红翠俞炯
Owner ZHEJIANG UNIV