Method for detecting surface difference membrane protein of mesenchymal stem cell of cord blood source

Abstract

The invention relates a method for detecting a difference membrane protein of a stem cell, and aims to provide a method for detecting the surface difference membrane protein of the mesenchymal stem cell of a cord blood source. The method comprises the following steps of: separating and cultivating the mesenchymal stem cell, extracting the membrane protein of the mesenchymal stem cell, obtaining amembrane protein gel graph by a two-directional fluorescence difference gel electrophoresis (2D-DIGE) method, and analyzing and checking the mass spectrum. In the method, the 2D-DIGE method is first used for detecting a special difference surface marker of the membrane surface of the stem cell so as to perform quantitative comparison and analysis on proteins in different samples on the same gel; an interior label is added into each gel; and DIA and BVA software can automatically calibrate an expression quantity of each protein point according to the interior label of the protein point, so theerror between gel and gel in different batches is reduced to the maximum extent, the real change degree of the protein is reflected, and the false positive rate and the false negative rate are lowered.

Description

field of invention [0001] The invention relates to a method for detecting differential membrane proteins of stem cells, in particular to a method for detecting differential membrane proteins on the surface of cord blood-derived mesenchymal stem cells (MSCSs) and placenta-derived mesenchymal stem cells. Background technique [0002] Stem cells (stem cells, SC) are a kind of pluripotent cells with self-replication ability, which can differentiate into various functional cells under certain conditions. Mesenchymal stem cells (MSCSs) are an important member of the stem cell family, originally derived from bone marrow, because of their strong proliferation ability and multi-lineage differentiation potential, hematopoietic support and promotion of stem cell implantation, immune regulation and other characteristics make them useful in tissue engineering, Cell therapy and gene therapy have broad clinical application prospects. In recent years, with the in-depth study of the biologi...

AI Technical Summary

Problems solved by technology

Among them, human umbilical cord blood MSCSs have extremely consistent biological characteristics, and may be more primitive, have stronger proliferation and differentiation capabilities, are easy to store, and have low immune reactivity