Chip and reagent for measuring special protein in urine
A protein chip and chip technology, applied in the direction of biological testing, material inspection products, etc., can solve the problems of inaccurate urine protein measurement results, changes in biochemical components, etc.
Inactive Publication Date: 2011-06-15
孙续国 +2
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Problems solved by technology
[0003] The technical problem solved by the present invention is to overcome the influence of complex components in the urine and the reaction environment on the results in the process of measuring urine protein using chip technology. Changes in the biochemical components contained in the test results in inaccurate urine protein determination results and the establishment of four special protein series combinations for the diagnosis of kidney damage, which prevents excessive detection from increasing the burden on patients and causes missed diagnosis due to too little detection, and improves The diagnostic rate of kidney damage
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[0020] On May 27, 2009, the title awarded to Mu Haidong was "Preparation of a Protein Chip for Detection of Multiple Tumor Markers in Males" announcement number: CN201247246Y. On October 4, 2006, the title awarded to Li Zesong et al. was "Hepatic Fibrosis Related Serum Marker Detection Protein Chip" publication number: CN1841068A. The preparation process of some antigen detection protein chips is disclosed in detail. The main steps are (1) prepare the nitrocellulose membrane; (2) design the chip, determine the dot array arrangement and spotting position; (3) suck out the antibody from the 384-well plate with the spotting needle and spray it on the nitrocellulose membrane (4) Assemble the nitrocellulose membrane, and carry out sealing, drying, packaging, and preservation.
[0021] Specific preparation steps
[0022] Determination of tumor marker detection antigen
[0023] 1 Determination of detection index antigen
[0024] The tumor marker CEA is composed of six highly simi...
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Abstract
The invention discloses a chip and a matched reagent suitable for measuring special protein in urine. The matched reagent is 1 mol chemical mixed solution which comprises Tris and Hcl, and the final pH value of 7.4. The chip comprises a chip base plate, chip frames and a nylon film, wherein the nylon film is sandwiched between two frames, the thickness of the frame close to the base plate is 3mm, and the thickness of the frame above the nylon film is 5mm; the lower frame is tightly adhered to the base plate, and windows in the frames have an equal size and are square or round; and the chip base plate is fixed with four antibodies, namely a transthyretin antibody, a beta-2 globulin antibody, an albumin antibody and an immunoglobulin G antibody. A patented project is that: 1, the chip for measuring the special protein in the urine is characterized in that: the nylon film between the matched reagent and the chip frames and a urine protein specific antibody marked on the base plate are combined; and 2, according to the claims (1), the chip for detecting urine protein is characterized in that: carrier groove windows of the chip frame (2) and the chip frame (4) are more than six (including six) single grooves formed in a square or round shape.
Description
technical field [0001] The invention relates to a protein chip and matching reagents for measuring special protein in urine. More specifically, the present invention designs the matching reagents for adjusting the urine environment reagents, increasing the stability and specificity of urine special protein determination results, and at the same time improves the structure of the chip board by adding a nylon film on the chip bottom plate frame. technical background [0002] When the glomeruli and renal tubules are damaged, the protein content in urine increases, and the degree of increase is related to the degree of kidney damage. The methods and reagents for measuring urine protein are widely used in clinical diagnosis. Therefore, the method of urine protein determination has attracted much attention. There are many quantitative methods for urine protein, such as yellow sulfuric acid method, Ponceau chromogenic method, and immune method, but these methods can only measure ...
Claims
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IPC IPC(8): G01N33/68
Inventor 孙续国
Owner 孙续国