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Establishing method for high frequency regeneration system of hemarthria compressa

A high-frequency regeneration technology for verbena tachycarpa, applied in plant regeneration, horticultural methods, botanical equipment and methods, etc., can solve problems such as inability to effectively complete follow-up operations, incomplete technical solutions, and lack of necessary descriptions, and achieve High reproducibility, simple operation, and easy-to-control effects

Inactive Publication Date: 2012-09-05
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the operation process disclosed in this document, the first step is to start the culture to obtain the callus, and the second step is to subculture the regenerated shoots. There is no necessary description of the cultivation process of the regenerated shoots obtained from the callus between the two steps. Therefore disclosed technical scheme is incomplete
Moreover, the tissue culture technique of Verbena flat tachycarpa disclosed in this document cannot be reproduced repeatedly, and there are technical difficulties in the operation of inducing callus in the first step of starting the culture stage, and it is impossible to effectively complete the follow-up operation

Method used

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  • Establishing method for high frequency regeneration system of hemarthria compressa
  • Establishing method for high frequency regeneration system of hemarthria compressa
  • Establishing method for high frequency regeneration system of hemarthria compressa

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Experimental program
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Effect test

Embodiment 1

[0014] like figure 1 As shown, the method of the present invention is used to establish a high-frequency regeneration system of Verbena flat tachycarp.

[0015] 1. Materials

[0016] (1) Plant explant material: take the young leaves of Verbena platyscens as the explant material.

[0017] (2) Main reagents:

[0018] 2,4-D (2,4-dichlorophenoxyacetic acid) mother solution: after dissolving 2,4-D with a small amount of absolute ethanol, add distilled water to make a 1mg / L mother solution, and store it at 4°C until use.

[0019] 6-BA (6-benzylaminopurine) mother solution: dissolve 6-BA with a small amount of 10% sodium hydroxide, add distilled water to make a 1mg / L mother solution, and store it at 4°C until use.

[0020] NAA (naphthalene acetic acid) mother solution: NAA was dissolved with a small amount of 10% sodium hydroxide, and distilled water was added to make a 1 mg / L mother solution, which was stored at 4°C until use.

[0021] (3) Basal medium: MS medium, the pH of MS m...

Embodiment 2

[0052] Establish the high-frequency regeneration system of Verbena flat tachycarpa with the inventive method, and it is no longer repeated with the same part as embodiment one, and its difference is:

[0053] (1) Step S1 callus induction

[0054] Start medium: MS medium + 2,4-D 0.5~2.5mg / L+6-BA 0~0.4mg / L

[0055] The callus was induced and cultured for 60 days, and the culture results are shown in Table 6.

[0056] Table 6 callus induction results

[0057]

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Abstract

The invention discloses an establishing method for a high frequency regeneration system of hemarthria compressa. The technology scheme of the organization culture system for the hemarthria compressa in the prior art is not complete or clear, or even the method has not necessary of the defect of repeat reproducibility. The invention provides the method for establishing the high frequency regeneration system by taking the hemarthria compressa young stem or young leaves as the explant. The method comprises the following steps of: taking the young stem or young leaves of the plant as the explant for establishing the high frequency regeneration system of the hemarthria compressa according to the operating processes of callus inducing, callus subculture, callus differentiation training, regrowth propagation culture, regrowth root training and regrowth seedlings transplanting, and optimizing the selection of the explant and the medium components of each cultivating stage for raising the throughput and product quality of each stage of the regeneration system. The method has reliable principle; and the established high frequency regeneration system of the hemarthria compressa has the characteristics of fast growth, high repeatability, simple operation, easy control on condition, and the like.

Description

technical field [0001] The invention relates to a method for establishing a high-frequency regeneration system of Verbena flat tachycarpa, belonging to the field of plant regeneration through tissue culture technology in agricultural cultivation. Background technique [0002] Verbena flat spikes (Hemarthria compressa (Linn.f.) R.Br) is a perennial herbaceous plant of the Poaceae Verbena genus. The stem is thin and soft, and the forage quality is good. Livestock likes to eat it. It can be used for grazing or drying hay. In addition, verbena has a well-developed root system, thick grass and large coverage, and is also a good soil and water conservation plant. The planting of Verbena flat tachycarpa can not only meet the needs of ecological environment construction, but also an important measure to promote the sustainable development of animal husbandry. [0003] The article "Establishment of the Tissue Culture System of Verbena scutellaria" published in "Journal of Grassland...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 杨春华张文君张新全刘琳陈灵鸷刘帆何凌斐
Owner SICHUAN AGRI UNIV