Preparation method of oat beta-glucan
A technology of dextran and oats, which is applied in the direction of anti-toxins, digestive system, metabolic diseases, etc., can solve the problems of unfavorable industrial production, instability, cumbersome process, etc., and achieve large-scale production, stable properties, and good solubility Effect
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Embodiment 1
[0014] The concrete preparation method of embodiment 1 is as follows:
[0015] (1) Low-temperature extraction: add oat bran to 0.01 mol / L NaOH solution at a mass ratio of 1:10, shake in a constant temperature water bath at 30°C for 2 hours. Water was added according to the mass ratio of oat bran to water at 1:15, passed through a 180-mesh sieve, centrifuged at 4000 rpm for 20 min, and the supernatant was poured into the oat bran.
[0016] (2) High-temperature leaching: adjust the pH value of the mixture to 10.0, shake in a constant temperature water bath at 80°C for 3 hours. Cool to room temperature, centrifuge at 4000rpm for 20min, and discard the precipitate.
[0017] (3) Pectinase and amylase treatment: adjust the pH value of the extract to 5.5, add pectinase 0.06mg / 5g oat bran, stir at 60°C for 5min, then inactivate the enzyme at 100°C for 5min, cool to room temperature, and centrifuge at 4000rpm for 20min , discard the precipitate. Adjust the pH of the extract to 6.5, ...
Embodiment 2
[0027] The concrete preparation method of embodiment 2 is as follows:
[0028] (1) Low-temperature extraction: add 0.01mol / L NaOH solution to oat bran at a mass ratio of 1:8, shake in a constant temperature water bath at 30°C for 3 hours; add water at a mass ratio of oat bran to water of 1:10, and pass through a 100-mesh sieve Net, centrifuged at 4000rpm for 30min, poured out the supernatant in oat bran;
[0029] (2) High-temperature leaching: adjust the pH value of the mixture to 10.0, shake in a constant temperature water bath at 80°C for 3 hours, cool to room temperature, centrifuge at 4000rpm for 20 minutes, and discard the precipitate;
[0030] (3) Pectinase and amylase treatment: adjust the pH value of the extract to 5, add pectinase 0.01mg / 5g oat bran, stir at 65°C for 30min, then inactivate the enzyme at 100°C for 5min, cool to room temperature, and centrifuge at 5000rpm for 25min , discard the precipitate; adjust the pH value of the extract to 5.5, add high temperatu...
Embodiment 3
[0035] The concrete preparation method of embodiment 3 is as follows:
[0036] (1) Low-temperature extraction: Add 0.05mol / L NaOH solution to oat bran at a mass ratio of 1:12, shake in a constant temperature water bath at 25°C for 1 hour; add water at a mass ratio of oat bran to water of 1:15, and pass through a 200-mesh sieve Net, centrifuged at 5000rpm for 15min, poured out the supernatant in oat bran;
[0037] (2) High-temperature leaching: adjust the pH value of the mixture to 10.0, shake in a constant temperature water bath at 80°C for 3 hours, cool to room temperature, centrifuge at 4000rpm for 20 minutes, and discard the precipitate;
[0038] (3) Pectinase and amylase treatment: adjust the pH value of the extract to 6, add pectinase 0.1mg / 5g oat bran, stir at 65°C for 5min, then inactivate the enzyme at 100°C for 5min, cool to room temperature, and centrifuge at 4000rpm for 15 ~30min, discard the precipitate; adjust the pH value of the extract to 6.5, add high temperat...
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