Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for detecting multiple transgenic plants based on silica spheres coded by fluorescence quantum dots (QDs)

A technology of silica spheres and fluorescent quantum dots, applied in chemical instruments and methods, fluorescence/phosphorescence, luminescent materials, etc., to achieve the effect of simple operation, regular shape, and good monodispersity

Inactive Publication Date: 2011-08-31
JIANGNAN UNIV
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, domestic and foreign studies are only focused on QDs-encoded polystyrene microspheres.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting multiple transgenic plants based on silica spheres coded by fluorescence quantum dots (QDs)
  • Method for detecting multiple transgenic plants based on silica spheres coded by fluorescence quantum dots (QDs)
  • Method for detecting multiple transgenic plants based on silica spheres coded by fluorescence quantum dots (QDs)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The 535nmQDs, 602nmQDs, 629nmQDs, and 650nmQDs quantum dots used were purchased from Wuhan Jiayuan Co., Ltd. The following different volume ratios were used:

[0049] 535nmQDs: 629nmQDs=2:1,

[0050] 535nmQDs: 629nmQDs=1:2,

[0051] 535nmQDs: 602nmQDs: 650nmQDs=1:1:2,

[0052] Preparation of 535nmQDs: 602nmQDs: 650nmQDs=1:2:2 coded silica spheres and their application in the detection of transgenic corn, soybean, rapeseed and cotton.

[0053] 1. Fabrication of fluorescent quantum dot-encoded silica spheres

[0054] (1) Mix 15 mL of Triton and 15 mL of n-hexanol for 10 minutes, add 60 mL of cyclohexane, and stir for 20 minutes.

[0055] (2) Mix 1400 μL 535nm QDs and 700 μL 629nm QDs evenly, add to the above solution, stir evenly for 30 min, and configure a microemulsion system.

[0056] (3) Add 2mL of ammonia water to the above system, mix for 30min, add 2mL of TEOS and 2mL of APTES, and hydrolyze for 12h.

[0057] (4) Add 15 mL of acetone to break the emulsion, s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for detecting multiple transgenic plants based on silica spheres coded by fluorescence quantum dots (QDs), belonging to the technical field of application of materials. The silica spheres prepared by adopting a reverse micro-emulsion method are uniform, the process is simple and easy to operate, the silica spheres can be accurately coded by controlling the types and the proportions of the QDs, the fluorescence of the QDs is protected by using silica, and the stability of the QDs is enhanced. By adopting the silica spheres coded by the fluorescence QDs, the aim of detecting multiple transgenic plants is successfully fulfilled.

Description

technical field [0001] A fluorescent quantum dot-based coded dioxide [0002] The invention relates to a method for detecting multiple transgenic plants with silicon balls, and belongs to the technical field of material application. Background technique [0003] Quantum dots, also known as nanocrystals, English name: quantum dots, QDs for short, are nanoparticles composed of II-VI or III-V elements with a particle size between 1 and 10nm. QDs have quantum size effect and surface effect, and can emit fluorescence after being excited. Compared with traditional fluorescent dyes, QDs have broad excitation spectrum, narrow emission spectrum and high fluorescence quantum yield. QDs have gradually replaced dyes as a new type of biomarker material, and are widely used in cell imaging, immunofluorescence, in vivo imaging, and nucleic acid recognition. [0004] Silicon dioxide, English name: silicon dioxide, is a transparent inert material. The synthesis technology of silica is qu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64C09K11/08
Inventor 王利兵胥传来赵媛陈伟郝昌龙
Owner JIANGNAN UNIV