Cell printing system capable of constructing three-dimensional human tissues in vitro
A technology of human tissue and cell printing, applied in tissue cell/virus culture devices, biological material sampling methods, bioreactors/fermenters for specific purposes, etc., can solve problems such as low cell survival rate, and achieve cell survival High efficiency, easy to use, high printing efficiency
Inactive Publication Date: 2011-09-07
XI AN JIAOTONG UNIV
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Problems solved by technology
After electrospray cell printing, the cell survival rate is low, about 70%, and the ejection of droplets is assisted by a high-voltage electric field. The voltage of up to several thousand volts is a potential threat to the operator.
Method used
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Embodiment Construction
[0013] The present invention will be described in detail below in conjunction with the accompanying drawings.
[0014] Referring to the accompanying drawings, a cell printing system capable of constructing three-dimensional human tissue in vitro, including a syringe 1, the top of the syringe 1 is connected to the gas outlet end of the nitrogen cylinder 3, when the nitrogen flows out, the liquid in the syringe 1 will be at a certain pressure Next, the switch of the cell printing head of the syringe 1 is connected to the signal output terminal of the pulse signal generator 2, and the pulse signal generator 2 controls the opening and closing of the cell printing head switch of the syringe 1, and the flow out of the cell printing head is in the open state. Nitrogen will drive the injector 1 to spray liquid droplets containing cells or continuous fluid. A three-dimensional mobile platform 4 is arranged below the injector 1. The reception of droplets is completed by the movement of t...
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Abstract
The invention relates to a cell printing system capable of constructing three-dimensional human tissues in vitro. The system comprises an injector, wherein the top of the injector is connected with the gas outlet end of a nitrogen cylinder; the switch of a cell printing head of the injector is connected with the signal output end of a pulse signal generator; a three-dimensional moving platform is arranged below the injector; liquid drops are received by moving the three-dimensional moving platform; the control signal input end of the three-dimensional moving platform is connected with a computer; during printing, the pulse signal generator controls the switching on and off state of the cell printing head of the injector; when the switch of the cell printing head is switched on, a part of liquid in the injector can form the liquid drops under the extrusion of nitrogen; the liquid drops are received by the three-dimensional moving platform; the computer is used for controlling the ordered movement of the three-dimensional moving platform; and various tissues or organs can be printed by changing the rheological property of cell suspension in the injector. The invention has the advantages that: the cell printing system is easy to construct and convenient to use.
Description
technical field [0001] The invention relates to the field of biotechnology, in particular to a cell printing system capable of constructing three-dimensional human tissues in vitro. Background technique [0002] Cell printing (Cell Bioprinting) is an advanced technology for constructing a three-dimensional multicellular system in vitro, which can solve problems that are difficult to solve in traditional tissue engineering, and has broad development prospects in biomedical basic and applied research. During the cell printing process, cells and cell culture fluid or cell-compatible biomaterials such as hydrogel precursors are placed in the nozzle of the printer at the same time, and the deposition position of the droplet containing cells is controlled by the computer, and printed point by point at the specified position. On the basis of printing one layer, continue to print another layer, layer by layer to form a three-dimensional multi-cell / gel system. The technologies curre...
Claims
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IPC IPC(8): C12M3/00
CPCC12M21/08C12M33/00
Inventor 徐峰周丽宏卢天健
Owner XI AN JIAOTONG UNIV