Method for establishing gas chromatography (GC) fingerprint of Pinus massoniana needle volatile oil component and application thereof

A technology of gas chromatography and masson pine needles is applied in the field of traditional Chinese medicine analysis, which can solve the problems of unloaded pine needles and the like, and achieve the effects of rapid method, good popularity of instruments, equipment and materials, and simple operation.

Inactive Publication Date: 2011-09-07
ZHEJIANG ACAD OF TRADITIONAL CHINESE MEDICINE
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AI Technical Summary

Problems solved by technology

[0004] In view of the fact that the standard of pine needles is not included in the Pharmacopoeia (2010 edi

Method used

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  • Method for establishing gas chromatography (GC) fingerprint of Pinus massoniana needle volatile oil component and application thereof
  • Method for establishing gas chromatography (GC) fingerprint of Pinus massoniana needle volatile oil component and application thereof
  • Method for establishing gas chromatography (GC) fingerprint of Pinus massoniana needle volatile oil component and application thereof

Examples

Experimental program
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Example Embodiment

[0038] Example 1

[0039] A method for establishing a gas chromatography-fingerprint pattern of the volatile oil from pinus massoniana needles. The method includes the following steps:

[0040] 1) Precisely draw and weigh 0.05mg bambooene reference substance, place it in a 5mL volumetric flask, add dichloromethane to the mark, shake well to prepare caryophyllene stock solution, and then accurately draw 0.5mL caryophyllene stock solution, and place In a 5mL volumetric flask, add dichloromethane to the mark, shake well, and then prepare a caryophyllene reference solution with a mass concentration of 1μg / mL;

[0041] 2) Collect pine needles (Lin'an, Zhejiang Province), cut into 2cm sections and dry in the shade, accurately weigh 50g of pine needles, put in a volatile oil extractor, add 1L of water, soak for 10h, according to the volatile oil extraction method (2010 edition Chinese Pharmacopoeia Appendix XD) , Add water from the upper end of the tester to fill the graduated part, connec...

Example Embodiment

[0046] Example 2

[0047] Collect 10 batches of pine needle medicinal materials from different origins, obtain gas chromatograms according to the above method, and then conduct GC analysis. Table 1 shows the relative retention time of the shared fingerprint peaks of 10 batches of pine needles; Table 2 shows the relative peak area of ​​the shared fingerprint peaks of 10 batches of pine needles.

[0048]

[0049]

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Abstract

The invention belongs to the technical field of traditional Chinese medicine analysis for identifying pine medicinal materials and comparing components, and in particular relates to method for establishing gas chromatography (GC) fingerprint of a Pinus massoniana needle volatile oil component and an application thereof. The method for establishing the GC fingerprint of the Pinus massoniana needle volatile oil component comprises the following steps: 1) preparing a reference solution; 2) preparing a test article solution; 3) performing gas chromatographic analysis; 4) calibrating the common peak in the GC fingerprint of Pinus massoniana needle volatile oil; and 5) obtaining the analysis result of the GC fingerprint of the Pinus massoniana needle volatile oil. Compared with the methods in literatures, the method related by the invention is fast, simple and convenient, and has the advantages that the repeatability and stability are both good; the characteristic is strong; the method can be used as a basis for identifying pine; and a basis for quality control of Pinus massoniana medicinal materials and preparations of the materials is provided.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine analysis, and is used for identification and component comparison of pine needle medicinal materials, and in particular relates to a method for establishing a gas chromatography (GC) fingerprint of pine needle volatile oil components and an application thereof. Background technique [0002] Masson pine (Pinus massoniana Lamb.) is the most widely distributed tree species in the humid subtropical south of my country. Dried masson pine needles are commonly used as a traditional Chinese medicine. It is recorded in "Compendium of Materia Medica": "Pine needles have a bitter, warm, and poisonous smell. Taking it for a long time makes people not old, lightens the body and nourishes qi, and cures rheumatic sores, grows hair, calms the five internal organs, and keeps the middle. , not hungry and prolong life." Modern research shows that pine needles have the effects of calming, analges...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 俞忠明寿旦张扬李洪玉章建民
Owner ZHEJIANG ACAD OF TRADITIONAL CHINESE MEDICINE
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