S1P lyase inhibitors for the treatment of cerebral malaria
A technology of lytic enzymes and inhibitors, applied in the field of treatment, compositions useful therefor, treatment and/or prevention of cerebral malaria, which can solve the problem of no future for treatment
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[0130] Various aspects of the present invention can be understood from the following examples, but are not limited to the scope of the following examples.
[0131] Synthesis of (E / Z)-1-(4-((1R,2S,3R)-1,2,3,4-tetrahydroxybutyl)-1H-imidazol-2-yl)-ethanone oxime
[0132]
[0133] 1-[4-((1R,2S,3R)-1,2,3,4-tetrahydroxy-butyl)-1H-imidazol-2-yl]-ethanone (THI, according to Halweg, K.M. and Büchi , G., J. Org. Chem. 50:1134-1136 (prepared in 1985)) (350mg, 1.52mmol) was suspended in water (10ml). Hydroxylamine hydrochloride (126.8 mg, 1.82 mmol, 1.2 eq.) and sodium acetate (247.3 mg, 3.04 mmol. 2 eq.) were added, and the suspension was stirred at 50°C. The reaction mixture became clear after about 4 hours. Stirring was continued at 50°C for 16 hours. LCMS analysis showed product formation and the absence of starting material. The reaction mixture was brought to room temperature and passed through a fine mesh filter. This solution was used directly to purify the product by pr...
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