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Star-shaped cationic polymer, preparation method thereof and application thereof

A cationic polymer and reaction technology, applied in the field of biomedical engineering materials, can solve problems such as accelerating ulcer healing, accelerating the degradation of growth factors, and aggravating local inflammatory reactions of wounds, achieving good water solubility, biocompatibility, and high transfection efficiency effect

Inactive Publication Date: 2011-09-28
SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(3) In patients with diabetic foot, local application of growth factors alone cannot accelerate ulcer healing. It may be related to the increase of MMP-9 to accelerate the degradation of growth factors, growth factor receptors, integrins and their receptors, thereby aggravating the wound local ulcer healing. Inflammatory response, slows wound healing
[0005] Gene transduction requires high-efficiency and high-safety vectors. Due to the potential safety issues of viral vectors, their application in vivo experiments is largely limited. Therefore, people have been working hard to find new gene transduction vectors.

Method used

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  • Star-shaped cationic polymer, preparation method thereof and application thereof
  • Star-shaped cationic polymer, preparation method thereof and application thereof
  • Star-shaped cationic polymer, preparation method thereof and application thereof

Examples

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Effect test

preparation Embodiment 1

[0042] 1. Synthesis of three generations of alkyne-containing polyamide-amine dendrimers

[0043] (1) Under ice-water bath, under nitrogen protection, dissolve 1.5g of propargylamine in 5mL of methanol, and slowly drop into methanol solution of methyl acrylate (7.03g of methyl acrylate in 10mL of methanol). After 2 hours of dropwise addition, the mixture was stirred at 0° C. for 1 hour, then warmed up to room temperature and stirred for 24 hours. After the reaction, unreacted methyl acrylate and methanol were removed by rotary evaporation, and vacuum-dried to obtain 0.5 generation polyamidoamine dendrimers with a yield of 95%.

[0044] (2) Under ice-water bath, under nitrogen protection, dissolve 5.4g of 0.5-generation polyamide-amine dendrimer in 20mL of methanol, and slowly drop into the methanol solution of ethylenediamine (28.5g of ethylenediamine dissolved in 30mL of methanol) . After 2 hours of dropwise addition, the reaction was first stirred at 0° C. for 1 hour, and ...

preparation Embodiment 2

[0051] 1. Synthesis of alkyne-containing polyamide-amine dendrimers with different alkyne groups

[0052] (1) Under ice-water bath, under nitrogen protection, dissolve 1.5g of propargylamine in 5mL of methanol, and slowly drop into the methanol solution of methyl acrylate (11.73g of methyl acrylate is dissolved in 10mL of methanol). After 2 hours of dropwise addition, the mixture was stirred at 0° C. for 2 hours, then warmed up to room temperature and stirred for 36 hours. After the reaction, unreacted methyl acrylate and methanol were removed by rotary evaporation, and vacuum-dried to obtain 0.5 generation polyamidoamine dendrimers with a yield of 98%.

[0053] (2) Under ice-water bath, under nitrogen protection, dissolve 4.54g of 0.5-generation polyamide-amine dendrimers in 15mL of methanol, and slowly drop into methanol solution of ethylenediamine (36.0g of ethylenediamine dissolved in 15mL of methanol) . After 2 hours of dropwise addition, the reaction was first stirred ...

preparation Embodiment 3

[0060] 1. Synthesis of three generations of alkyne-containing polyamide-amine dendrimers

[0061] (1) Under ice-water bath, under nitrogen protection, dissolve 1.5g of propargylamine in 5mL of methanol, and slowly drop into methanol solution of methyl acrylate (9.0g of methyl acrylate in 10mL of methanol). After 2 hours of dropwise addition, the mixture was first stirred at 0° C. for 1.5 hours, then warmed up to room temperature and stirred for 30 hours. After the reaction, unreacted methyl acrylate and methanol were removed by rotary evaporation, and vacuum-dried to obtain 0.5 generation polyamidoamine dendrimers with a yield of 95%.

[0062] (2) Under ice-water bath, under nitrogen protection, dissolve 5.0g of 0.5-generation polyamide-amine dendrimers in 20mL of methanol, and slowly drop into the methanol solution of ethylenediamine (33.0g of ethylenediamine dissolved in 30mL of methanol) . After 2 hours of dropwise addition, the reaction was first stirred at 0° C. for 1.5...

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Abstract

The invention discloses a star-shaped cationic polymer beta-CD-PAMAM and a preparation method thereof. The method comprises the following steps: putting the beta-CD-(N3)7 and PAMAM in a mixed solvent of t-butyl alcohol and water; stirring to dissolve; dropping an aqueous solution of copper sulfate pentahydrate and sodium ascorbate into the mixed solvent; heating to a temperature of 40 to 50 DEG C, and carrying out a reaction for 24 to 28 hours with stirring in nitrogen atmosphere; dialyzing a reaction product by a dialysis bag; filtering; and carrying out freeze drying. So the beta-CD-PAMAM is obtained. The invention also provides an application that the beta-CD-PAMAM is treated as a gene transduction carrier in interfering with MMP-9 expressing by RNA. According to the invention, the star-shaped cationic polymer has good water solubility and biocompatibility; the polymer has a high transfection efficiency and the polymer can form the nanometer particles with siRNA, because a large amount of surface cationic groups can form nanometer particles with the siRNA; and the surface cationic groups provide the possibility of further functionalization.

Description

technical field [0001] The invention belongs to the field of biomedical engineering materials, in particular to a star cationic polymer and its preparation method, and its application as a gene transduction carrier in the expression of RNA interference MMP-9. Background technique [0002] Diabetics have fragile skin and it is difficult to heal wounds once they occur. The reasons mainly include: (1) systemic factors of the patient (such as metabolic nutritional status, immunity, etc.); (2) local factors of the wound (such as whether there is co-infection, vascular disease of surrounding tissues, neuropathy, etc.); (3) microscopic wound Changes (excessive secretion of matrix metalloproteinases, lack of growth factors, decreased tissue proliferation, abnormal microcirculation, excessive secretion of inflammatory factors, etc.). These reasons eventually lead to an imbalance of extracellular matrix synthesis and degradation, thereby affecting ulcer healing. [0003] Matrix meta...

Claims

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Application Information

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IPC IPC(8): C08G81/00C08G69/00C08B37/16C12N15/63C12N15/113
Inventor 杨川严励张黎明邓俊杰李娜
Owner SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV