Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for preparing oat ACE (Angiotensin Converting Enzyme) inhibitory peptide by using enzymatic membrane reactor

An enzyme membrane reactor and a technology for inhibiting peptides are applied in the field of preparing ACE inhibitory peptides from oats, which can solve the problems of unstable product performance, long production cycle, high labor intensity, etc., and achieve the effect of good application prospects.

Inactive Publication Date: 2011-10-12
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the industrial hydrolysis of protein to prepare biologically active peptides is almost all intermittent production, which has many shortcomings, such as one-time use of enzymes, unstable performance of each batch of products, long production cycle and high labor intensity, etc. The use of enzyme membrane reactor can well overcome these shortcomings

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing oat ACE (Angiotensin Converting Enzyme) inhibitory peptide by using enzymatic membrane reactor
  • Method for preparing oat ACE (Angiotensin Converting Enzyme) inhibitory peptide by using enzymatic membrane reactor
  • Method for preparing oat ACE (Angiotensin Converting Enzyme) inhibitory peptide by using enzymatic membrane reactor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1, the influence of enzyme membrane reactor system on enzyme activity

[0040] First, add 2500ml buffer solution (0.02mol / L pH value = 8.0 phosphate buffer) to the reaction tank, and add 12.5ml Alcalase, stir for 15min, take 5ml enzyme solution to measure its enzyme activity and absorbance at 280nm; then, turn off the ultra- Open the peristaltic pump at the permeate outlet of the filter membrane. The flow rate of the feed liquid is 25L / h. After 3 minutes of normal temperature and normal pressure circulation, open the outlet of the ultrafiltration membrane permeate and adjust the pressure to 0.01MPa. Add phosphate buffer solution to the system, record the amount added, open the pressure control valve, turn off the peristaltic pump, close the permeate outlet after each cycle for 60 minutes, measure the volume of the ultrafiltration membrane permeate, and measure its enzyme activity and 280nm Absorbance; then, add a certain amount to the system by adding the amo...

Embodiment 2

[0043] Embodiment 2, investigate the main factors affecting enzyme activity in the enzyme membrane reactor system

[0044] 1) Effects of peristaltic pump, agitator, pressure gauge and silicone tube on enzyme activity

[0045] First, remove the ultrafiltration membrane in the enzyme membrane reactor, and use the reaction tank, peristaltic pump, agitator, pressure gauge and silica gel tube to form a circulation system; then, add 2000ml buffer solution (0.02mol / L pH value =8.0 phosphate buffer), and add 10mlAlcalase, stir for 15min, take 5ml of enzyme solution and measure its enzyme activity and absorbance at 280nm; then, turn on the peristaltic pump, feed liquid flow rate is 25L / h, normal temperature, normal pressure cycle, every cycle 60min , take 5ml of enzyme solution to measure its enzyme activity and absorbance at 280nm; finally, turn off the peristaltic pump, divide the enzyme solution in the system into two equal parts, one part is placed at room temperature for 5 hours, ...

Embodiment 3

[0052] Embodiment 3, enzyme membrane reactor prepares ACE inhibitory peptide

[0053] 1) Design of the test plan

[0054] Firstly, the DPS (Data Processing System) software was used to design a homogeneous experiment to study the effects of the six factors of substrate concentration, enzyme amount, feed liquid flow rate, operating pressure, temperature and pH value on the enzyme activity retention rate, polypeptide production efficiency and ACE inhibition rate. Then, use SPSS 16.0 for window software to process the data, analyze the degree of influence and primary and secondary order of the six factors on each index; then, use DPS software to process the data, and find out when the enzyme activity retention rate and polypeptide production efficiency take the maximum value. The optimal combination level of factors; finally, based on the optimal combination level of each factor when the enzyme activity retention rate and polypeptide production efficiency take the maximum value, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
volumeaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for preparing oat ACE (Angiotensin Converting Enzyme) inhibitory peptide. Proved by research on the influence of an enzymatic membrane reactor system to enzyme activation, the enzymatic membrane reactor system has a good preservation effect to Alcalase, wherein Alcalase enzyme activation loss are mainly resulted from enzyme damage by an operation process of a peristaltic pump and enzyme absorption by an ultra-filtration membrane. By designing a four-factor three-level response surface analysis test by using Design Expert 6.0.5, the optimal process condition for preparing the oat ACE inhibitory peptide by using the enzymatic membrane reactor is as follows: the concentration of a substrate is 3%; enzyme addition quantity is 19900 U / g of oat protein; the flowrate of material liquid is 45 L / h; the operation pressure is 0.07 MPa; the temperature is 61 DEG C; the pH value is 8; and the ACE inhibition ratio of a product under the condition can be up to 67.86%.

Description

technical field [0001] The invention relates to a method for preparing oat ACE inhibitory peptide by using an enzyme membrane reactor. Background technique [0002] Hypertension is the main cause of complications such as coronary heart disease, myocardial infarction, stroke, and renal failure. It is known as the "first killer" of human health. Angiotensin Converting Enzyme (ACE) is the A key enzyme that increases blood pressure (Ondetti MA, Rubin B, Cushman DW. Design of specific inhibitors of angiotensin converting enzyme: a new class of orally active antihypertensive agents [J]. Science, 1997(196): 441-444.) , the purpose of preventing and treating hypertension can be achieved by inhibiting the activity of ACE. At present, ACE inhibitors are mainly used for the treatment of hypertension, which are mainly chemically synthesized. Although they have high activity and specificity, they will cause serious toxic and side effects during administration (Messerli F H. Combinations...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
Inventor 任清张晓平郭项雨
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY