Denitrifying bacterial strain and application of the same in water body for nitrogen removal together with wetland plants

A denitrifying bacteria and denitrification technology, applied in the field of environmental microorganisms, can solve the problems of little research and achieve stable denitrification effect, easy to cultivate, and good practical application prospects

Active Publication Date: 2011-10-19
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the denitrification and denitrification technology mainly focuses on the development of new processes and concepts of biological denitrification technology, microbial community structure in sewage treatmen

Method used

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  • Denitrifying bacterial strain and application of the same in water body for nitrogen removal together with wetland plants
  • Denitrifying bacterial strain and application of the same in water body for nitrogen removal together with wetland plants
  • Denitrifying bacterial strain and application of the same in water body for nitrogen removal together with wetland plants

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0033] Example 1 Isolation and identification of bacterial strains of the present invention:

[0034] (1) Culture medium:

[0035] A. Strain isolation, purification and preservation medium ( / L): CH 3 COONa, 2g; Peptone, 15g; Yeast extract, 3g; Glucose, 1g; NaCl, 6g; KNO 3 , 1.5g; pH controlled at 7.0~7.2.

[0036] B. Strain screening, denitrifying medium (DM: Denitrifying Medium) ( / L): CH 3 COONa, 2g; KH 2 PO 4 , 0.4g; MgSO 4 ·7H 2 O, 0.6 g; CaCl 2 ·2H 2 O, 0.07g; KNO 3 , 1g; Tris buffer 12mL; trace elements 2mL; pH controlled at 7.0 ~ 7.2.

[0037] If the above-mentioned medium is made into a solid medium, 1.5% agar is added.

[0038] (2) Separation and purification of Paenibacillus XP1:

[0039] Weigh 10 g of the soil sample in the rhizosphere of Rhizoma chinensis and place it in a 250 mL conical flask, add 100 mL of sterile water and a few glass beads, and shake on a shaker for 15 min to disperse the soil sample evenly. After the soil sample was dispersed, let ...

Example Embodiment

[0055] The cultivation of embodiment 2 strain of the present invention

[0056] (1) Medium used

[0057] A, strain preservation medium ( / L): CH 3 COONa, 2g; peptone, 15g; yeast extract, 3g; glucose, 1g; NaCl, 6g; agar, 12g; KNO 3 , 1.5g; pH controlled at 7.0~7.2.

[0058] B. Strain denitrifying medium (DM: Denitrifying Medium) ( / L): CH 3 COONa, 2g; KH 2 PO 4 , 0.4g; MgSO 4 ·7H 2 O, 0.6 g; CaCl 2 ·2H 2 O, 0.07g; KNO 3 , 1g; Tris buffer 12mL; trace elements 2mL; pH controlled at 7.0 ~ 7.2.

[0059] If the above-mentioned medium is made into a solid medium, 1.5% agar is added. Sterilize at 121°C for 20 minutes before use.

[0060] (2) Culture conditions

[0061] The Paenibacillus XP1 stored on the slant of the preservation medium was scraped with an inoculating loop to scrape a ring of bacterial fur, and inoculated into a 250 mL conical flask containing 200 mL of preservation medium or denitrification medium, and cultured in a 30°C constant temperature incubator. 12...

Example Embodiment

[0062] Embodiment 3 The denitrification performance of the strain of the present invention under optimum denitrification conditions

[0063] The experiment was carried out in 250mL conical flasks, each containing 100mL denitrification wastewater, and the content of various substances in the wastewater was adjusted to make XP1 in the best denitrification conditions required for denitrification. join OD 600 =0.1(cm -1 ), 10mL bacterial liquid, 30 ℃, 120r / min culture, regularly detect the OD of bacteria in the medium 600 and total nitrogen concentration. The experimental results see Image 6 . from Image 6 It can be seen that the lag phase of strain XP1 is not obvious, and it enters the exponential growth phase after 8 hours, and the OD 600 It increased from 0.15 to 1.35. After 50 hours, it entered the stationary phase, and the XP1 strain tended to decline after 90 hours. OD 600 Gradually decreased. The total nitrogen removal rate increased continuously with the growth o...

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Abstract

The invention discloses a denitrifying bacterial strain, which is named Paenibacillus sp.XP1, belongs to paenibacillus of paenibacillus ash, and has been preserved in China Center for Type Culture Collection on April 10, 2011, wherein the preservation number is CCTCC M2011120. The denitrifying bacterial strain can effectively minimize total nitrogen concentration in water, is applicable to the treatment of waste water having high concentration of nitrite or nitrate, and has wide adaptability. The process of treating waste water with the bacterial strain is simple and a stable effect of nitrogen removal is obtained. When the strain is used together with reed wetland plants, giant reed wetland plants and cattail wetland plants for nitrogen removal and simulated nitrogen containing waste water has a total nitrogen concentration of 50 mg/L, the three XP1 bacteria liquid reinforced wetlands all have a substantially improved removal rate of nitrogen, and reinforced nitrogen removal effect that the XP1 bacteria liquid exerts on the cattail wetland is obviously better than the effect exerted on the reed wetland and the giant reed wetland; when the XP1 bacteria liquid stays for 96 hours, an XP1 bacteria liquid reinforced cattail wetland has a removal rate of nitrogen of 94%, while an ordinary cattail wetland has a removal rate of nitrogen of 14%.

Description

technical field [0001] The invention relates to a denitrifying bacterium and its application in synergistic denitrification with wetland plants in water bodies, belonging to the field of environmental microorganisms. Background technique [0002] With the increasing eutrophication of water bodies, the existence of nitrogen, especially ammonia nitrogen and nitrite nitrogen, has a certain poisonous effect on many aquatic animals. Removing nitrogen pollution in water has become a hot issue in the field of water pollution prevention and control. Constructed wetland technology has been widely used in sewage treatment because of its advantages of easy establishment, easy management, low cost, low energy consumption, and conducive to ecological restoration. Studies have shown that the absorption of plants in wetlands is very limited, and its contribution rate generally does not exceed 10% of the TN removal rate. The removal of nitrogen in constructed wetlands mainly depends on the ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F3/28C12R1/01
Inventor 裴海燕胡文容邵媛媛
Owner SHANDONG UNIV
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