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Polysaccharide from Eucommia ulmoides leaves with anti-complement activity, method of extracting, separating and purifying the same

An anti-complement and Eucommia leaf technology, applied in anti-inflammatory agents, non-central analgesics, antipyretics, etc.

Inactive Publication Date: 2011-10-26
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This patent technology does not separate and purify Eucommia leaf polysaccharides

Method used

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  • Polysaccharide from Eucommia ulmoides leaves with anti-complement activity, method of extracting, separating and purifying the same
  • Polysaccharide from Eucommia ulmoides leaves with anti-complement activity, method of extracting, separating and purifying the same
  • Polysaccharide from Eucommia ulmoides leaves with anti-complement activity, method of extracting, separating and purifying the same

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Embodiment

[0023] 1 Extraction and content determination of Eucommia leaf polysaccharide

[0024] Grind the dried eucommia leaves by hand into pieces of <5mm (because it contains a lot of eucommia gum), add a certain volume of petroleum ether, reflux at 80°C and magnetically stir for 2 hours to remove the surface fat and eucommia gum, repeat once , take the filter residue, suction filter, air-dried, extract with hot water, add water to submerge the raw material, extract with boiling reflux for 2 hours, extract three times, squeeze and filter with three layers of gauze to remove the residue, combine the extracts, and concentrate under reduced pressure to a specific gravity of 1.1-1.2. Mix the polysaccharide concentrate with an equal amount of absolute ethanol, statically precipitate, and centrifuge to obtain 1 volume of alcohol-precipitated polysaccharide PsEUL1; separate the supernatant, add 1 volume of ethanol, mix well, and obtain 2 volumes of alcohol-precipitated polysaccharide PsEUL2 ...

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Abstract

The invention discloses a polysaccharide from Eucommia ulmoides leaves with anti-complement activity, and its method of extracting, separating and purifying, wherein, the polysaccharide from Eucommia ulmoides leaves with anti-complement activity is an acidic polysaccharide. The extraction method comprises the following steps: adding eucommia fragments in petroleum ether, reflowing and magnetic stirring, and extracting filtration; air drying the residues, then using hot water for boiling-refluxing extraction, filtering and removing the residues with gauze; extracting the extract, carrying out decompression condense; mixing the polysaccharide concentrate with waterless ethanol, standing for precipitation, and using centrifugation to obtain a alcohol precipitated polysaccharide. The separating and purifying method comprises the following steps: producing a polysaccharide solution from crude polysaccharide, extracting filtration, and using macroporous resin to decolour; removing protein by using Sevag method, mixing Sevag reagent with the polysaccharide sample uniformly, carrying out centrifugation, collecting supernatant and condensing, UV-scanning until no absorption peak; respectively dialyzing the crude polysaccharide solution; weighing polysaccharide to dissolve in distilled water, and eluting on a cellulose column to obtain the monomer component of refined polysaccharide. The method realizes the exploitation of folium cortex eucommiae resource, lays a foundation for the application in pharmacy industry, and serves the health of human.

Description

technical field [0001] The invention relates to polysaccharides of organic macromolecule compounds, in particular to eucommia leaf polysaccharides, and also relates to its extraction, separation and purification methods. Background technique [0002] After the 1960s, people gradually discovered that polysaccharides have special biologically active functions, and found that many polysaccharides and their derivatives have medicinal value, especially in anticoagulant, antithrombotic, blood lipid regulation, immune function regulation and Both anti-tumor and anti-radiation aspects have significant pharmacological effects and curative effects. In terms of anti-complement activity, polysaccharides of many higher plants can activate complement, and have a certain balance inhibitory effect on the overactivation of the complement system caused by inflammation. Eucommia ulmoides leaves polysaccharide (polysaccharide from Eucommia ulmoides leaves, PsEUL) is one of the above polysaccha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P29/00
Inventor 张学俊宫本红龚桂珍
Owner GUIZHOU UNIV
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