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Purification method of alpha-mangostin

A technology of mangostin and ethanol solution, which is applied in the field of natural product separation, can solve the problems of complex process, high content, simple process, etc., and achieve the effect of easy process and safe operation

Inactive Publication Date: 2011-11-16
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As mentioned above, there are two problems in the existing method of purifying α-mangostin: one is high content but complex process; the other is simple process but low content

Method used

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  • Purification method of alpha-mangostin

Examples

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Comparison scheme
Effect test

Embodiment 1

[0018] Take the mangosteen shell and crush it, weigh 10kg, add 10 times the amount of 80% ethanol solution, heat to 60°C, soak for 2 hours, filter the liquid, add 3 times the amount of 80% ethanol, heat to 60°C for 2 hours, extract twice, The combined filtrates were concentrated under reduced pressure to obtain a concentrate. Take ethanol, water, and ammonium sulfate, mix them in a ratio of 4:5:5, add the concentrated solution to tube centrifugation, collect the organic phase and concentrate, add water to the concentrated solution, add 2L polyamide column for adsorption, and use 8L 30% ethanol solution → 12L 50% in turn Elute with ethanol solution, detect with TLC, collect high-concentration fractions, concentrate under reduced pressure, and place to crystallize. Take petroleum ether, ethyl acetate, methanol, and water, mix them according to the ratio of 4:6:5:3, take the upper phase solution to dissolve the crude crystals, reflux to saturate and dissolve, filter and place the...

Embodiment 2

[0020] Take the mangosteen shell and crush it, weigh 10kg, add 10 times the amount of 60% ethanol solution, heat to 50°C, soak for 3 hours, extract twice, combine the filtrates and concentrate under reduced pressure to obtain a concentrate. Take ethanol, water, and ammonium sulfate, mix them in a ratio of 4:5:5, add the concentrated solution to tube centrifugation, collect the organic phase and concentrate, add water to the concentrated solution, add 2L polyamide column for adsorption, and use 10L of 20% ethanol solution → 8L of 70% in turn Elute with ethanol solution, detect with TLC, collect high-concentration fractions, concentrate under reduced pressure, and place to crystallize. Take petroleum ether, ethyl acetate, methanol, and water, mix them according to the ratio of 3:5:5:3, take the upper phase solution to dissolve the crude crystals, reflux to saturate and dissolve, filter and place the crystals, recrystallize 3 times, filter out the crystals and dry them , 232g of ...

Embodiment 3

[0022] Take mangosteen shells and crush them, weigh 10kg, add 8 times the amount of 70% ethanol solution, heat to 50°C, soak for 3 hours, extract twice, combine the filtrates and concentrate under reduced pressure to obtain a concentrate. Take ethanol, water, and ammonium sulfate, mix them in a ratio of 4:5:5, add the concentrated solution to tube centrifugation, collect the organic phase and concentrate, add water to the concentrated solution, add 2L polyamide column for adsorption, and use 10L of 30% ethanol solution → 9L of 60% Elute with ethanol solution, detect with TLC, collect high-concentration fractions, concentrate under reduced pressure, and place to crystallize. Take petroleum ether, ethyl acetate, methanol, and water, mix them according to the ratio of 3:6:5:3, take the upper phase solution to dissolve the crude crystals, reflux to saturate and dissolve, filter and place the crystals, recrystallize twice, filter the crystals and dry them , 218g of yellow powder wa...

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Abstract

The invention belongs to the field of natural product isolation and relates to a purification method of alpha-mangostin. The purification method comprises the following steps: 1) crushing mangosteen shells, adding 3-10 times of a 60-80% ethanol solution to soak and extract for 2-3 times, concentrating the extract while depressurizing, adding the concentrated solution into an aqueous two-phase solvent system for extraction, collecting the organic phase, and concentrating the collected organic phase to obtain a concentrate; 2) adding water to the concentrate for dispersion, adding the dispersed concentrate to a polyamide column for adsorption, carrying out gradient elution with an ethanol solution, detecting by thin-layer chromatography, collecting the eluate, concentrating the eluate while depressurizing, and standing for crystallization; and 3) filtering out the crystals, dissolving the crystals with a mixed solution, recrystallizing, and drying to obtain alpha-mangostin. The purification method of alpha-mangostin is simple in process and easy to operate, has high product purity, and is easy to enlarge production.

Description

Technical field: [0001] The invention belongs to the field of separation of natural products, and relates to a method for purifying α-mangostin. Background technique: [0002] α-mangostin (α-mangostin, α-MAG), commonly known as α-mangostin, chemical name 1,3,6-trihydroxy-7-methoxy-2,8 bis(3-methyl-2- Butene)-9-butanone, molecular formula C 24 h 26 o 6 , molecular weight 410.47, molecular structure formula: [0003] [0004] Mangosteen is the fruit of the genus Garcinia, also known as mangosteen, mangosteen, and pineapple. It is a tropical fruit. Mangosteen husk can treat dysentery, malaria, sprains, typhoid, ulcers, skin infections, anti-inflammatory and bactericidal and help wound healing traditional folk medicine. Among them, the active ingredient α-mangostin has antibacterial, anti-inflammatory, anti-oxidation, anti-cancer and anti-AIDS effects, and has attracted people's attention in recent years. [0005] Existing methods for extracting and separating α-mangost...

Claims

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Application Information

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IPC IPC(8): C07D311/86
Inventor 刘东锋杨成东
Owner NANJING ZELANG MEDICAL TECH
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