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Microbial composite inoculum and applications thereof

A technology of compound inoculants and microbial strains, applied in the direction of microorganisms, microorganisms, biocides, etc., can solve the problems of human and animal insecurity, high toxicity, environmental pollution, etc.

Inactive Publication Date: 2012-01-18
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional chemical nematicides are mostly highly toxic and residual organophosphorus and carbamate pesticides, which seriously pollute the environment and are not safe for humans and animals

Method used

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  • Microbial composite inoculum and applications thereof
  • Microbial composite inoculum and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: Control tomato root-knot nematode test with bacterial agent

[0036] Transplant 1-month-old tomatoes (4-6 leaves), the tomato variety is: Improved Maofen 802, produced by Shandong Agricultural Science and Technology Development Company. Apply 100ml of bacterial agent to each pot, and carry out the control effect test according to the above test method. The list shows the anti-efficacy data.

[0037] The control effect (60d) of table 1 bacterial agent to tomato root-knot nematode disease

[0038]

[0039] The results showed that the fungal agent had significant control effect on tomato root-knot nematode.

Embodiment 2

[0040] Embodiment 2: the control effect comparison of different bacterial agents

[0041] The method of embodiment 2 is the same as that of embodiment 1, the difference is: the application of ZK7 bacterial agent is 100ml per pot, and the application of B16 bacterial agent is 100ml per pot.

[0042] The synergistic effect of table 2 bacterial agent

[0043]

[0044] The results show that when applied for 90 days, the control effect of the bacterial agent of the present invention is increased by 31.3% compared with the single application of the ZK7 bacterial agent, and is 28.4% higher than that of the single application of the B16 bacterial agent.

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Abstract

The invention discloses a microbial composite inoculum and applications thereof, belonging to the technical field of microbial pesticides. The microbial composite inoculum provided by the invention is prepared by a method comprising the following steps: selecting microbial bacterial strain verticillium chilmaydosporium ZK7 with the preservation number CGMCC No. 0418 and bacillus nematocidus B16 with the preservation number CGMCC No.1128 which are preserved in the common microbial center of China committee for culture collection of microorganisms; respectively preparing a ZK7 spore suspension and a B16 bacteria suspension according to the conventional method; and then mixing according to the mass ratio of (0.5-2)-(0.2-1), thus a liquid inoculum is obtained. The microbial composite inoculum prepared by the invention is prepared for the applications of a preparation for preventing and treating plant nematode. The microbial composite inoculum provided by the invention has the advantages of high efficiency, low toxicity and residue free, and achieves room temperature prevention of more than 90% for tomato root knot nematode disease.

Description

Technical field: [0001] The invention relates to a microbial compound bacterial agent and its application, belonging to the technical field of microbial pesticides. technical background: [0002] Root-knot nematode (Meloidogyne spp.) is a soil-dwelling endoparasitic nematode, which can harm vegetables, especially melons and solanaceous vegetables, with an annual yield loss of 30% to 50%. Meanwhile, root-knot nematode disease has aggravated wilting The occurrence of soil-borne diseases such as root rot and root rot has become a major obstacle in current vegetable production. Chemical soil treatment is one of the important measures to control root-knot nematodes. Most of the traditional chemical nematicides are highly toxic and highly residual organophosphorus and carbamate pesticides, which seriously pollute the environment and are not safe for humans and animals. Among them, many nematicides have been banned one after another, and the development of new and efficient soil ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/14C12N1/20A01N63/02A01N63/04A01P5/00C12R1/07C12R1/645
Inventor 刘亚君乔敏邹顺惺张克勤
Owner YUNNAN UNIV
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