Check patentability & draft patents in minutes with Patsnap Eureka AI!

Preparation method for L-ornithine-alpha-ketoglutarate

A technology of ketoglutarate and ornithine, applied in directions such as being fixed on/in organic carriers, fermentation, etc., can solve problems such as increased production cost, reduced product yield, unfavorable product separation and purification, etc., to achieve production The effect of low cost, less impurities and safe production operation

Inactive Publication Date: 2013-08-07
湖南天成生化科技有限公司
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Because the above-mentioned L-ornithinase production process uses free cells to transform the substrate L-arginine, so the transformation liquid will contain a small amount of bacterial protein and other impurities, which is not conducive to the separation and purification of the product; and the transformation must be removed. Bacteria, reducing product yield, resulting in increased production costs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A method for producing L-ornithine-α-ketoglutarate by enzymatic conversion, characterized in that the method comprises the steps of:

[0031] (1) Preparation of immobilized enzyme

[0032] The amount of raw materials is as follows: 50g of triethanolamine anionic styrene resin, 400mg of arginase, and saturated saline subject to being able to submerge and immobilize the enzyme, take 50g of triethanolamine anionic styrene resin, soak it in 2M potassium carbonate solution for 12h, filter, After filtering to dryness, add 100ml 2M potassium carbonate solution, add 10ml hydrogen bromide (2g / ml) under ice bath and react for 15min under ice bath, wherein the concentration of triethanolamine anion styrene resin is 40g / ml. Wash with cold 0.1M sodium bicarbonate until the pH value reaches 8.5, add 100ml of arginase solution (4g / l by weight), and react at 4°C for 24h. Wash with 0.1M sodium bicarbonate, 1M sodium chloride, and deionized water three times in sequence, 200ml each time...

Embodiment 2

[0038] (1) Preparation of immobilized enzyme

[0039] Take 40g of triethanolamine anionic styrene resin, soak in 1M potassium carbonate solution for 8h, filter and dry, add 10ml of 1M potassium carbonate solution, add 5ml of hydrogen bromide (1g / ml) under ice bath and react for 5min under ice bath , wash with cold 0.05M sodium bicarbonate until the pH value reaches 7, add 400ml of arginase solution (weight percent concentration is 1g / l) and react at 1°C for 12h. Wash with 100ml of 0.05M sodium bicarbonate solution, 100ml of 0.5M sodium chloride solution, and 100ml of deionized water for 2 times, and finally soak the immobilized enzyme in saturated saline for 0.5h, and wash until no chloride ion is detected in the solution. Obtain immobilized enzyme, store it in water, and set aside;

[0040] (2) Optimization of transformation conditions

[0041] Arginine 60g, acetic acid 6.4g and manganese acetate 0.2g were mixed, dissolved in 90g deionized water to obtain a mixed solution, ...

Embodiment 3

[0045] (1) Preparation of immobilized enzyme

[0046]Take 50g of triethanolamine anionic styrene resin, soak in 2M potassium carbonate solution for 12h, filter and dry, add 100ml of 2M potassium carbonate solution, 10ml of hydrogen bromide (2g / ml) and react in ice bath for 15min, then use cold 0.1M Wash with cold sodium bicarbonate until the pH value reaches 8.5, add 100ml of arginase solution (4g / l by weight) and react at 4°C for 24h. Wash with 200ml of 0.1M sodium bicarbonate solution, 200ml of 1M sodium chloride solution, and 200ml of deionized water for 3 times, and finally soak the immobilized enzyme in saturated saline for 1 hour, and wash until no chloride ion is detected in the solution to prepare the immobilized enzyme. Catase, stored in water, for subsequent use;

[0047] (2) Optimization of transformation conditions

[0048] 60 g of arginine, 7 g of acetic acid and 0.2 g of manganese acetate were mixed, dissolved in 90 g of deionized water, and 50.4 g of the immob...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a preparation method for L-ornithine-alpha-ketoglutarate. The L-ornithine-alpha-ketoglutarate is produced by arginase transformation. The preparation method comprises the following steps: (1) preparation of immobilized enzyme; (2) optimization of transformation conditions; (3) product extraction and refining process. Compared to the prior art, the preparation method of the present invention has the following advantages that: the preparation method of the present invention has characteristics of low production cost, mild production conditions, less impurities in the transformation system, simple process steps, safe production operation and high purity; qualified products meeting the light transmission requirements can be synthesized; each liter of the reaction solution contains 300-320 g of the L-ornithine-alpha-ketoglutarate, and the transformation efficiency of the alpha-ketoglutaric acid is more than 90%; the preparation method further has other advantages.

Description

technical field [0001] The invention relates to a preparation method of amino acid salt, in particular to a preparation method of L-ornithine-α-ketoglutarate. Background technique [0002] L-Ornithine-α-ketoglutarate is a raw material widely used in medicine and health care. It is a muscle energy mixture, and it can also help accelerate the growth of children with growth hormone deficiency and accelerate the wound healing of trauma patients. [0003] The method for preparing L-ornithine-α-ketoglutarate is mainly to crystallize after direct salt-forming reaction between L-ornithine and α-ketoglutarate. However, due to the different preparation methods of L-ornithine, L-ornithine-α-ketoglutarate can be prepared in different ways. [0004] At present, there are many reports on the preparation of L-ornithine at home and abroad. There are three main methods for preparing L-ornithine: chemical method, fermentation method, and enzymatic method. [0005] Chemical method: At presen...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/02C12N11/02
Inventor 苏建勇郑南华张建宇
Owner 湖南天成生化科技有限公司
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com