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Method for preparing chitosan compound nano/micron capsule with core-shell structure

A core-shell structure and chitosan technology, applied in the direction of microcapsule preparation, microsphere preparation, etc., can solve the problems of low encapsulation efficiency and entrapment capacity, no microcapsule structure, easy to occur adhesion, etc., and achieve convenient operation , The preparation technology is simple and the effect of good economic development potential

Active Publication Date: 2012-04-11
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In existing research inventions, it has been reported that a single chitosan nanoparticle has been prepared by ion cross-linking, which can carry hydrophilic drugs and has good drug sustained release effect; however, the prepared chitosan nanoparticles However, it is also reported that carboxymethyl chitosan nanoparticles, a water-soluble derivative of chitosan, are used as hydrophilic drug carriers, but due to the negative charge on their surface, they are not easy to be phagocytized by cells, reducing the Intracellular drug delivery efficiency of carboxymethyl chitosan nanoparticles
Pure chitosan nanoparticles and carboxymethyl chitosan nanoparticles do not have a microcapsule structure, and the encapsulation of drugs is mainly through entanglement and adsorption, so the encapsulation efficiency and encapsulation capacity are low

Method used

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  • Method for preparing chitosan compound nano/micron capsule with core-shell structure
  • Method for preparing chitosan compound nano/micron capsule with core-shell structure
  • Method for preparing chitosan compound nano/micron capsule with core-shell structure

Examples

Experimental program
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Effect test

Embodiment 1

[0018] Prepare O-carboxymethyl chitosan with reference to the method of Chen and Park 2003; Get the chitosan 50mg that molecular weight is 1200kDa, be dissolved in 10ml 1% (v / v) hydrochloric acid solution, drip into 5ml 0.84% ​​hydrochloric acid solution under stirring condition (v / v) sodium tripolyphosphate solution, dripped in 20 minutes, stirred at room temperature at 100 rpm for 40 minutes, added dropwise 20ml of O-carboxymethyl chitosan containing 0.25% (w / v) under stirring Solution, dripped in 30 minutes, adjusted pH to pH 4 with sodium hydroxide, stirred at room temperature, stirred at 200 rpm for 5 hours, centrifuged at 12000 rpm for 1 hour, removed the supernatant, and freeze-dried to obtain chitosan composite sodium / micron capsules.

Embodiment 2

[0020] Prepare O-carboxymethyl chitosan with reference to the method of Chen and Park 2003; Get the chitosan 100mg that molecular weight is 500kDa, be dissolved in 10ml 1% (v / v) hydrochloric acid solution, drop into 5ml 0.96% hydrochloric acid solution under stirring condition (v / v) sodium tripolyphosphate solution, dripped in 20 minutes, stirred at 100 rpm at room temperature for 40 minutes, added dropwise 20ml of O-carboxymethyl chitosan containing 0.5% (w / v) under stirring Solution, dripped in 30 minutes, adjusted pH to pH 4 with sodium hydroxide, stirred at room temperature, stirred at 200 rpm for 5 hours, centrifuged at 12000 rpm for 1 hour, removed the supernatant, and freeze-dried to obtain chitosan composite sodium / micron capsules.

Embodiment 3

[0022] With reference to Miao, the method of Chen et al.2006 prepares N, O-carboxymethyl chitosan; Get the chitosan 200mg that molecular weight is 19.6kDa, be dissolved in 10ml 1% (v / v) acetic acid solution, under stirring condition Add dropwise 5ml of 0.42% (w / v) sodium tripolyphosphate solution, drop it in 20 minutes, stir at room temperature at 100 rpm for 40 minutes, add dropwise 20ml of 1% (w / v) O- Carboxymethyl chitosan solution, dripped in 30 minutes, adjusted the pH to pH 5 with sodium hydroxide, stirred at room temperature, stirred at 200 rpm for 5 hours, centrifuged at 12000 rpm for 1 hour, removed the supernatant, and freeze-dried To obtain chitosan composite nano / micro capsules.

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Abstract

The invention discloses a technology for preparing a nano / micron capsule, namely a method for preparing a chitosan compound nano / micron capsule, belonging to the field of high polymer material science. In the method, chitosan is taken as a raw material, and a target product can be synthesized through a three-step method comprising chitosan carboxymethylation, ion crosslinking and polymer condensation. The method is implemented as the following steps of: performing carboxymethylation on the chitosan to obtain a carboxymethyl chitosan and chitosan solution which is crosslinked with sodium tripolyphosphate at the same time; and then dripping carboxymethyl chitosan in the sodium tripolyphosphate-chitosan solution to perform polymer condensation to obtain a chitosan nano / micron capsule with a core-shell structure, wherein the particle size of the capsule is 80-1800nm, and Zeta level is between -18.3 and -34.5mv. The chitosan compound nano / micron capsule with a core-shell structure prepared by the method in the invention can be used as a medical biological material and a drug carrier, and has good search, development and utilization prospects.

Description

technical field [0001] The invention belongs to the field of biomaterials and relates to a preparation method of chitosan composite nano / micro capsules with a core-shell structure. Background technique [0002] Chitosan is a natural cationic biopolysaccharide, which has the advantages of large amount of resources, biodegradability, good stability, good biocompatibility, safety and non-toxicity, etc. It is a good resource for the development of biomedical materials. In existing research inventions, it has been reported that a single chitosan nanoparticle has been prepared by ion cross-linking, which can carry hydrophilic drugs and has good drug sustained release effect; however, the prepared chitosan nanoparticles However, it is also reported that carboxymethyl chitosan nanoparticles, a water-soluble derivative of chitosan, are used as hydrophilic drug carriers, but due to the negative charge on their surface, they are not easy to be phagocytized by cells, reducing the The e...

Claims

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Application Information

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IPC IPC(8): B01J13/14
Inventor 陈西广冯超程晓杰
Owner OCEAN UNIV OF CHINA