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Method for determining floating percentage of microcystis

A microcystis, percentage technology, applied in the field of determining the floating percentage of Microcystis, can solve the problem of limiting the outbreak mechanism of cyanobacteria blooms, achieve the effects of short sinking and floating time, easy operation, and reduced errors

Inactive Publication Date: 2013-08-28
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there is still a lack of an accurate method for determining the percentage of flotation of unicellular algae grown indoors, which limits the research on the mechanism of cyanobacterial blooms.

Method used

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  • Method for determining floating percentage of microcystis
  • Method for determining floating percentage of microcystis
  • Method for determining floating percentage of microcystis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The culture temperature is 25°C, and the light intensity is 100umol / m 2 Microcystis under the condition of s is transferred to a set temperature of 10°C and a light intensity of 0umol / m 2 • Culture in the light incubator of s, take a sample every two hours, and measure the change of the floating percentage of Microcystis over time. The specific operation is as follows: put the pipette tip in an autoclave, sterilize it at 120°C for 30 minutes, then put it in the ultra-clean workbench, put the pipette tip in the ultra-clean workbench and sterilize it with ultraviolet light for 30 minutes, wait for After the tip of the pipette is cooled, take samples in the ultra-clean workbench according to the method of aseptic operation. Shake the algae well before sampling, then use a pipette to draw a small amount of algae solution, and drop a drop on the cell counting plate (CELL-VU). Cover the edge of the glass to slowly infiltrate the algae liquid, absorb the excess algae liquid w...

Embodiment 2

[0053] The culture temperature is 25°C, and the light intensity is 100umol / m 2 Microcystis under the condition of s is transferred to a set temperature of 20°C and a light intensity of 100umol / m 2 Cultivate in the light incubator of s, take a sample once every two hours, measure the change of the floating percentage of Microcystis over time, the method of measuring the floating percentage is the same as above, the experimental results are shown in Table 3, as can be seen, under this condition , the floating percentage of Microcystis gradually decreased.

[0054] Table 3 Microcystis floating percentage changes

[0055]

Embodiment 3

[0057] The culture temperature is 25°C, and the light intensity is 100umol / m 2 Microcystis under the condition of s is transferred to a set temperature of 20°C and a light intensity of 500umol / m 2 Cultivate in the light incubator of s, take a sample every two hours, measure the change of the floating percentage of Microcystis over time, the method of measuring the floating percentage is the same as above, the experimental results are shown in Table 4, as can be seen, under this condition , the floating percentage of Microcystis decreased rapidly.

[0058] Table 4 Microcystis floating percentage changes

[0059]

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Abstract

The invention relates to a method for determining floating percentage of microcystis. According to the method, a BG11 culture medium is prepared, and is sterilized; the sterilized BG11 culture medium is placed in a superclean bench and cooled; a sterile operation method is adopted to inoculate microcystis, wherein the inoculation amount is 10<4>-10<5> cells / mL; then the inoculated medium is placed in an illumination incubator with a set temperature of 25 DEG C and light intensity of 100 mumol / m<2>.s to carry out culture; after the microcystis grows to the logarithmic phase, the microcystis isconveyed to an illumination incubator with a certain temperature and a certain light intensity to carry out culture, and is taken once in a while, wherein the experimental temperature range is 0-30 DEG C, the light intensity range is 0-500 mumol / m<2>.s, and a certain temperature and a certain light intensity are required by the experiment; the microcystis liquid is sucked by a pipette gun, and a drip of the microcystis liquid is added in a cell counting plate, wherein the counting plate is the cell counting plate (CELL-VU), and the depth of the counting chamber of the cell counting plate is 0.02 mm; the cell counting plate is placed under the microscope, and the counting treatments are respectively performed on the floating microcystis and the total algae.

Description

technical field [0001] The invention relates to a method for measuring the floating percentage of microcystis, which is helpful for studying the outbreak mechanism of cyanobacteria blooms, and belongs to the research field of prevention and control mechanism of water environment pollution. Background technique [0002] With the rapid development of industry and agriculture in my country and the acceleration of urbanization, a large amount of industrial wastewater, domestic sewage, and agricultural sewage containing N, P and other nutrients are discharged into water bodies, making lakes in China generally present with eutrophication. In the past 10 years, water pollution and eutrophication have occurred in many parts of the country. According to the 2010 China Environmental Status Bulletin, among the 26 state-controlled key lakes (reservoirs), 1 was severely eutrophic, accounting for 3.8%; 2 were moderately eutrophic, accounting for 7.7%; 11 were mildly eutrophic. one, accou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/14G01N15/04C12Q1/06
Inventor 李正魁周涛赵琳吴宁梅
Owner NANJING UNIV