Method for structuring lactate bio-enzyme electrode based on tetrapod-like nano zinc oxide
A four-needle nanometer, zinc oxide technology, which is applied in the direction of material analysis, measuring device, and instrument by electromagnetic means, can solve the problems of lowering the detection limit, low sensitivity, and low detection limit, etc. The method is simple and feasible, good repeatability
Inactive Publication Date: 2014-01-01
UNIV OF SCI & TECH BEIJING
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Problems solved by technology
[0005] The purpose of the present invention is to solve the current problem of low sensitivity and low detection limit in the field of lactic acid detection. By improving the enzyme electrode preparation process, the enzyme electrode is constructed, the sensitivity is greatly improved, and the detection limit is reduced.
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[0026] The performance of the lactic acid bio-enzyme electrode prepared under the following electrode preparation process is the best:
[0027] Firstly, a commercial standard gold electrode was polished, ultrasonically cleaned with alcohol and deionized water respectively, and the electrode was blown dry with high-purity nitrogen gas for use.
[0028] The surface of the prepared gold electrode is vertically fixed on the base, and the Nafion solution available for ion exchange is dropped on the surface of the cleaned gold electrode, and the surface of the electrode is modified to enhance the stability and adsorption of the electrode.
[0029]Disperse the T-ZnO with good crystallinity in the alcohol solution to form a suspension, drop the suspension onto the Nafion-modified electrode surface, and drop it again after the alcohol is fully volatilized, and use a polytetrafluoroethylene mold to control the T-ZnO The thickness and shape of the layer are added dropwise until T-ZnO for...
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Abstract
The invention discloses a method for structuring a lactate bio-enzyme electrode based on tetrapod-like nano zinc oxide, belonging to the field of structuring nano-material devices. The method is characterized by comprising the steps of: polishing a standard gold electrode, and ultrasonically cleaning the standard gold electrode completely with alcohol and deionized water; dripping a Nafion solution on the surface of the cleaned gold electrode so as to modify the surface of the electrode to enhance the stability of the electrode; dispersing T-ZnO in an alcohol solution to form a suspension, dripping the suspension on the surface of the electrode, dripping again when the alcohol is evaporated, controlling the thickness and shape of the T-ZnO by using a mould until a film layer is formed on the surface of the electrode by dripping the T-ZnO; dripping a phosphate buffered solution (PBS) to wet the T-ZnO layer, and dripping a lactate oxidase solution onto the T-ZnO layer after the PBS is evaporated; and finally dripping the Nafion solution on the surface of the electrode, and placing the electrode in a refrigerator for cryopreservation for later use after a film is formed. The method has the advantages that the lactate bio-enzyme electrode based on the T-ZnO is structured for the first time by using an adsorption method, the method is simple and feasible and is good in repeatability, and the enzyme electrode has the characteristics of high sensitivity, low linear range lower limit, favorable apparent Michaelis constant and high response speed, and the lactic acid determination in a low-concentration range is realized.
Description
technical field [0001] The invention belongs to the technical field of nanomaterial device construction, and in particular provides a method for constructing a four-needle nano-zinc oxide lactic acid biological enzyme electrode, which realizes the measurement of lactic acid within a certain concentration (especially low concentration) range. Background technique [0002] As one of the important indicators of human health, lactic acid content has already attracted widespread attention and a lot of research has been done. It is a metabolite of continuous muscle activity, and excessive accumulation means fatigue. It causes soreness and fatigue in the initial stage, if left untreated for a long time, it will cause acidification of the body, which may cause serious diseases. The determination of lactic acid is beneficial to basic metabolic research and exercise physiology research, and has applications in clinical diagnosis, food processing and storage, and scientific fitness. ...
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IPC IPC(8): G01N27/327
Inventor 张跃雷洋罗宁闫小琴宋宇刘羲
Owner UNIV OF SCI & TECH BEIJING