Microbiological degradation method for nitrobenzene industrial wastewater
A technology for microbial degradation and industrial wastewater, applied in the field of biological treatment of pollutants, can solve problems such as short survival time, low ability to resist the impact load of influent water quality, and incomplete treatment
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Embodiment 1
[0024] This example treats the nitrobenzene-containing wastewater produced in the production process of a certain pharmaceutical factory in Southwest China. The concentrations of CODcr and nitrobenzene are 2500-5800mg / L and 65-150mg / L respectively, the influent water is 200 tons / day, and the main biological process is a batch SBR reactor.
[0025] see figure 1 , using the compound bacterial liquid of Pseudomonas, Stenotrophomonas maltophilia, Acinetobacter baumannii, Hydrospirilla, Acidovorax avenae, Alcaligenes faecalis and Bacillus to carry out domestication culture, three Level expansion training. After the initial addition of 10 tons of compound bacterial agent, the reactor was kept running stably for 20 days, and the treated effluent CODcr<100mg / L, nitrobenzene content<0.05mg / L, the effect was good.
Embodiment 2
[0027] The composite bacterial agent used in Example 1 was evaluated in a microbiology laboratory of a certain university, and its main evaluation route is shown in the attached figure 2 .
[0028] The simulated wastewater in the laboratory is a pharmaceutical factory wastewater sample with a nitrobenzene content of 216mg / L and a CODcr of about 14,000. The biological treatment reactor used is a batch SBR reactor with a daily treatment volume of 40L and a residence time of 4h (1h Oxygen mixing, continuous aeration for 1 hour, static precipitation for 1 hour, water outlet for 1 hour), after treatment, the effluent CODcr<100mg / L, nitrobenzene concentration<0.05mg / L.
[0029] The biological factor analysis of the mixed solution in the reactor by molecular biology shows that the main dominant bacterial groups in the mixed solution are: Pseudomonas, Stenotrophomonas, Acinetobacter, and metamorphic snail Bacteria, Pseudomonas avenatus, Pseudomonas perforinosa, Pseudomonas pseudoalc...
Embodiment 3
[0031] In this example, 1) to 3) verify the treatment effect of the simulated wastewater without a single strain of the compound bacterial agent. 4) ~ 7) Verify the simulated wastewater treatment effect of a single strain.
[0032] The simulated wastewater in the laboratory is a pharmaceutical factory wastewater sample with a nitrobenzene content of 300mg / L and a CODcr of about 20,000. The biological treatment reactor used is a batch SBR reactor with a daily treatment volume of 40L and a residence time of 4h (1h Facultative aerobic mixing, continuous aeration for 1 hour, static precipitation for 1 hour, water outlet for 1 hour)
[0033] 1) Compound bacterial agent Pseudomonas
[0034] After treatment, the effluent CODcr=18564.46mg / L, and the concentration of nitrobenzene=48.46mg / L.
[0035] 2) Compound bacterial agent Stenotrophomonas maltophilia
[0036] After treatment, effluent CODcr=17668.00mg / L, nitrobenzene concentration=186.34mg / L.
[0037] 3) Acinetobacter baumanni...
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