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Cell culture apparatus for evaluating cell migration behavior

A technology of cell culture and cell migration, applied in the field of cell culture apparatus, can solve problems such as data inaccuracy, and achieve the effect of simple structure

Inactive Publication Date: 2012-06-27
王秀梅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are two defects in this cell culture device. One is that it can only compare the changes in cell behavior in the two matrix environments.
Second, the influence of gravity will lead to inaccurate data

Method used

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  • Cell culture apparatus for evaluating cell migration behavior
  • Cell culture apparatus for evaluating cell migration behavior
  • Cell culture apparatus for evaluating cell migration behavior

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] Such as figure 1 As shown, the present invention is composed of 7 regular hexagonal prism-shaped hollow cavities, the hollow cavities are used as cell culture units, one of the hollow cavities is located at the center of the present invention, and the remaining 6 hollow cavities and the central hollow cavities are shared with each other The wall filter membrane has a symmetrical honeycomb structure in appearance. Among them, the wall filter membrane is made of transparent polymer plastic: HA, CM or PCF. The pore size of the membrane has different selectivity, and the pore size can allow cells to pass through. The front view of its structure is as follows figure 2 shown. The bottom of the present invention is composed of an ultrafiltration membrane with cell retention, which can provide a nutrient and material exchange channel between the cell culture unit and the external medium, such as image 3 shown. The present invention has multiple size standards, and can be l...

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Abstract

The invention relates to a cell culture apparatus, which is characterized in that: regularly-hexagonal prism cell culturing cells are distributed symmetrically in a honeycomb form. Due to the adoption of the cell culture apparatus, the orientation behavior and transfer behavior of cells responding to different micro environments can be detected simply and efficiently.

Description

technical field [0001] The invention relates to a cell culture device in biological experiments, in particular to a novel plug-in cell culture device for analyzing and comparing cell migration behavior in different culture microenvironments. Background technique [0002] The in vitro culture analysis of living cells is an important work in the fields of biomedical experiments and medical pharmacy. At present, the method for culturing cells in vitro is to use cell culture dishes, plates and bottles made of glass or plastic polymer materials as containers, and inoculate cells in the above-mentioned containers filled with culture fluid for cultivation. With the development of tissue engineering and biomedical technology, cell culture methods are becoming more and more diversified. For example: culture on some gel materials, or three-dimensional cell culture on scaffolds composited with different materials, etc. [0003] The study of cell behavior is of great significance to t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/00
CPCC12M23/34C12M23/44C12M23/02
Inventor 王秀梅王琼
Owner 王秀梅