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Anti-tumor angiogenesis aptamer molecule and preparation method thereof

An angiogenesis and anti-tumor technology, applied in the field of anti-tumor angiogenesis aptamer molecules and its preparation, can solve the problems of complex operation, low efficiency, no systematic and mature methods, etc.

Active Publication Date: 2015-05-20
HUAQIAO UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the production of circularized aptamers is mainly through the method of in vitro enzyme ligation and the use of plasmids to produce circularized aptamers in vivo, which has the problems of low efficiency and complicated operation.
There is no systematic and mature method for how to maintain the specific recognition and binding properties of the nucleic acid aptamer molecules screened by SELEX to ligands and improve their stability in vivo

Method used

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  • Anti-tumor angiogenesis aptamer molecule and preparation method thereof
  • Anti-tumor angiogenesis aptamer molecule and preparation method thereof
  • Anti-tumor angiogenesis aptamer molecule and preparation method thereof

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Embodiment 1

[0048] 1. Cyclization aptamer molecular design

[0049] According to the aptamer sequence VE5a (HijiriHasegawa, Oxford University Press (2007), Nucleic Acids Symposium Series No.51: 399-400; Biotechnol Lett (2008) 30: 829-834) of VEGF165 screened by SELEX technology, the redesigned cyclic Vl nucleic acid aptamer sequence (V5aE+): its sequence is 5′-ACTTCCAGTCTATTCAATTGGGCCCGTCCGTATGGTGGGTGTGCGTGGCCACGAGTTTTAAAAAAAAAAAACCAAGCTTTTTTTTGATCATGT-3′. Such as figure 1 As shown in 1A and 1B, the linear aptamer V5aE screened by SELEX technology relies on three characteristic stem-loop structures to specifically recognize and bind the target molecule protein VEGF165 (such as figure 1 Marked by the solid circle in A), if the V5aE sequence is directly circularized without modifying the V5aE sequence, the characteristic stem-loop structure that recognizes the target molecule sequence disappears ( figure 1 B), so it is necessary to make some base modifications to the non-core sequence of ...

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Abstract

The invention discloses an anti-tumor angiogenesis aptamer molecule and a preparation method thereof. The anti-tumor angiogenesis aptamer molecule is characterized in that the sequence of the anti-tumor angiogenesis aptamer molecule is 5'-ACTTCCAGTCTATTCAATTGGGCCCGTCCGTATGGTGGGTGTGCGTGGCCACGAGTTTTAAAAAAAAAAACCAAGCTTTTTTTGATCATGT-3'. Cyclization modification design and preparation method processes which are performed aiming at linear deoxyribonucleic acid (DNA) aptamer molecules are built, and a method for efficiently and rapidly preparing annular DNA aptamers at a low cost is built through modified annular DNA aptamer molecules generated by RAC reaction.

Description

technical field [0001] The invention relates to an aptamer molecule for anti-tumor angiogenesis and a preparation method. Background technique [0002] Aptamers are DNA or RNA fragments that function equivalent to antibodies, can specifically recognize and bind target molecules, and effectively inhibit the biological activity of target molecules. The nucleic acid sequence information of the aptamer can be screened through the systematic evolution of ligand exponential enrichment (SELEX), and the aptamer molecule can be synthesized in large quantities and at low cost by chemical methods. DNA aptamer molecules are very stable in vitro, and can be stored at room temperature for more than one year in a lyophilized powder state, so they have good prospects for drug development. At present, aptamers are considered to be one of the most promising nucleic acid drugs, and the other three are RNAi, ribozymes and microRNA. Aptamers are effective when they interact with extracellular ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/115C12N15/10A61P35/00
Inventor 杨会勇刁勇林俊生杨丽
Owner HUAQIAO UNIVERSITY