Preparation method of high-strength porous chitosan microcarrier
A technology of porous chitosan and chitosan microspheres, applied in biochemical equipment and methods, microorganisms, tissue culture, etc., can solve the problems of low porosity and poor mechanical properties, and achieve low cost and high roundness , the effect of simple operation
Inactive Publication Date: 2013-09-25
UNIV OF SHANGHAI FOR SCI & TECH
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[0004] One of purpose of the present invention provides a kind of preparation of high-strength porous chitosan microcarrier in order to overcome the shortcoming that porosity is low when preparing chitosan microcarrier with chitosan as raw material poor mechanical properties method
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Embodiment 1
[0045] A preparation method of high-strength porous chitosan microcarrier, comprising the steps of:
[0046] (1) Preparation of solution
[0047] ①. Prepare 2.5% (W / V) chitosan acid solution
[0048] First measure 1ml of acetic acid and add it to a 100ml volumetric flask to make up the volume to obtain a 1% (V / V) acetic acid solution, then weigh 2.5g of chitosan and add it to the 1% (V / V) acetic acid solution;
[0049] ②, preparation of chitosan silica gel mixture
[0050] According to the mass ratio of silica gel to chitosan, that is, silica gel: chitosan is 3:1, weigh 7.5g of silica gel, add it to 2.5% (W / V) chitosan acid solution and stir evenly;
[0051] ③. Prepare 4.5% (W / V) sodium polyphosphate solution
[0052] Weigh 4.5g sodium polyphosphate and dissolve in 100ml deionized water;
[0053] ④, preparation of 5.0% (W / V) sodium hydroxide solution
[0054] Weigh 5.0g of sodium hydroxide and dissolve in 100ml of deionized water;
[0055] (2), high-voltage pulse into a ...
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Abstract
The invention discloses a preparation method of a high-strength porous chitosan microcarrier. The method comprises the following steps: manufacturing holes by using silica gel, wherein chitosan serves as a raw material; preparing chitosan microspheres containing the silica gel by using a high-voltage pulse microcapsule molding instrument under the high-voltage electrostatic action; precipitating the chitosan microspheres containing the silica gel in sodium polyphosphate solution for 5 hours; washing with distilled water; soaking the chitosan microspheres containing the silica gel into a sodium hydroxide solution at the temperature of 80 DEG C; dissolving the silica gel of the chitosan microspheres containing the silica gel; washing with deionized water; freeze-drying to obtain the porous chitosan microcarrier; and performing microwave radiation treatment to finally obtain the high-strength porous chitosan microcarrier. According to the high-strength porous chitosan microcarrier, the grain size is 400 to 700 microns, the aperture is 20 to 60 microns and the porosity is 93 percent. The high-strength porous chitosan microcarrier can be applied to suspension culture of adherent cells. The preparation method has the characteristics of simplicity and quickness in operation, low cost and good effect.
Description
technical field [0001] The invention belongs to the field of preparation of porous materials, in particular to a method for preparing a high-strength porous chitosan microcarrier that can be used for suspension culture of adherent cells. Background technique [0002] The heating of materials by microwaves belongs to "body heating", which is different from general surface heating. In theory, body heating enables materials to be heated evenly. Microwave technology has been successfully used to crosslink carboxymethyl chitosan membranes, enabling their application in wound therapy. The chitosan film is dried by microwave technology, which is 6 times faster than the traditional air convection heating method. [0003] Natural polymer materials are the earliest medical materials used by humans. They have good biocompatibility, almost all of them are degradable, and the degradation products are non-toxic. Among tissue engineering scaffold materials, typical natural polymer materi...
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IPC IPC(8): C08J9/28C08J9/26C08J3/28C08J3/24C08L5/08C12N5/02
Inventor 李琳刘宝林韩宝三刘连军李济宁
Owner UNIV OF SHANGHAI FOR SCI & TECH