Preparation and detection method for heat shock apoptosis intracranial glioma cells

Abstract

The invention provides a preparation and detection method for heat shock apoptosis intracranial glioma cells. The preparation and detection method is applied to a process for preparing a heat shock apoptosis intracranial glioma supported dendritic cell tumor vaccine. The process for preparing the heat shock apoptosis intracranial glioma supported dendritic cell tumor vaccine comprises the following steps of: allowing tumor cells to be in a heat shock state, and introducing the cells to perform overexpression of heat shock protein (HSP); introducing cell apoptosis by using a chemical medicine so as to prepare apoptosis cell antigen which is rich in HSP; and performing mixed culture on the prepared antigen and immature dendritic cells (DC) to obtain mature DC vaccine. The DC vaccine which is prepared by a heat shock method through supporting is high in maturity and can be used for introducing immune response.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for preparing and detecting heat-shock apoptotic glioma cells, which is applied to the preparation process of heat-shock apoptotic glioma-loaded dendritic cell tumor vaccines. Background technique [0002] Brain malignant glioma is the most common primary malignant tumor of the central nervous system. Due to the rapid growth and infiltrative growth along the nerve fibers, complete surgical resection is difficult; meanwhile, it is only moderately sensitive to chemotherapy and radiotherapy. Therefore, the current internationally recognized sequential treatment plan of conventional surgery, radiotherapy, and chemotherapy is difficult to cure brain malignant glioma. Immunotherapy is to achieve the real purpose of cure by adjusting and improving the function of the body's immune system and eliminating residual tumor cells. Currently, DC-based cell vaccine therapy is an active i...

AI Technical Summary

Problems solved by technology

Due to the rapid growth and infiltrative growth along the nerve fibers, it is difficult to completely remove it by surgery; at the same time, it is only moderately sensitive to chemotherapy and radiotherapy

Therefore, the current internationally recognized sequential treatment plan of conventional surgery, radiotherapy, and chemotherapy is difficult to cure malignant glioma of the brain.

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