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Method for promoting eel grass seeds to germinate and grow

A kind of bitter grass and seed technology, applied in the direction of seed and rhizome treatment, application, agriculture, etc., can solve the problems of yield, reproductive ability, decreased viability, long culture period of bitter grass culture, limited survival rate during the cultivation process, etc. The effect of improving the rate of photosynthesis, leaf growth, and germination rate

Inactive Publication Date: 2013-12-25
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bittergrass has strong water purification ability and strong tolerance to water pollution. It plays a very important role in the restoration and reconstruction of aquatic ecosystems. However, due to the limitation of water quality and the biological characteristics of aquatic plants Due to its biological characteristics, the seedling rate of bitter grass seeds is very low, and large-scale production cannot be formed. Therefore, in the current prior art, tissue culture methods are often used to promote the rapid propagation of bitter grass, such as:
[0003] Chinese patent 200410066029.6 takes the fertile organ rhizome of Erythrina fragrans, and through a series of sterilization processes, cultivates the aseptic seedlings of Erythria fragrans in the culture medium, and then puts the aseptic seedlings in the culture medium to induce germination. Heavy metal mercury salts are used for sterilization, which is likely to cause environmental pollution. In addition, the survival rate of sterile vaccines after sterilization is only over 60%.
[0004] Chinese patent 2004100606272 uses the subterranean shoot tip of Erythrina fragrans as a culture, sterilizes the culture of Erythrina fragrans, induces culture in induction medium, differentiates and proliferates, and takes root culture to propagate Erythria fragrans, and the survival rate is not disclosed in this method
In this method, the culture period of the Elichrysophyllum culture is longer. In addition, the composition of the induction medium is too complicated, which increases the cost. Moreover, heavy metal mercury salts are also used in this method, which is likely to cause environmental pollution.
[0005] In addition, the scale of tissue culture of Erythrina fragrans is limited, the survival rate of the culture process is also limited, and there is no variation process in the culture process, so if there are too many generations, the yield, reproductive ability, and vitality will decline.

Method used

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  • Method for promoting eel grass seeds to germinate and grow
  • Method for promoting eel grass seeds to germinate and grow
  • Method for promoting eel grass seeds to germinate and grow

Examples

Experimental program
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Effect test

Embodiment 1

[0031] The temperature of the incubator is 20-30° C., and the light intensity is 1200 lux. Add distilled water, 2mg / L, 4mg / L, 8mg / L, 15mg / L and 30mg / L potassium nitrate solutions, and 0.25mg / L, 0.5mg / L, 1mg / L shells into 9 incubators respectively polysaccharide solution, each incubator was supplemented with micro-intermittent aeration, and 50 bitter grass seeds were put into each incubator and soaked for 12 hours; The grass seeds were cultivated and the results are shown in Table 1.

[0032] Table 1 Germination time and germination rate of bitter grass seeds soaked in different solutions

[0033]

Embodiment 2

[0035] The temperature of the incubator is 20-30° C., and the light intensity is 2500 lux. Add 8mg / L potassium nitrate solution into the incubator, supplemented by micro intermittent aeration at the same time, put 50 bitter grass seeds into the incubator, and soak for 22 hours; After the bitter grass seeds are cultivated, the germination time of the bitter grass seeds is 4 to 11 days, and the germination rate is 100%.

Embodiment 3

[0037] The temperature of the constant temperature incubator was kept constant at 20-30° C., and the light intensity was 2800 lux. Add 0.25mg / L chitosan solution in the constant temperature incubator, supplemented by micro-intermittent aeration at the same time in the constant temperature incubator, put 50 bitter grass seeds and soak for 36 hours; The soaked bitter grass seeds are cultivated in the beaker, the germination time of the bitter grass seeds is 5-12 days, and the germination rate is 88%.

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Abstract

The invention relates to a method for promoting eel grass seeds to germinate and grow. The method comprises the following steps that: in a culture box, potassium nitrate solution or chitosan solution is added, in addition, trace intermittent aeration is used as an auxiliary measure, and the eel grass seeds are added for soaking; the soaked eel grass seeds are cultured in a beaker with lake bottom sediment as substrates; and after the eel grass seeds germinate, the young eel grass seedlings are placed into a culture box containing the potassium nitrate to be soaked, and meanwhile, trace intermittent aeration and intermittent short wave ultraviolet ray radiation are used as auxiliary measures. The method provided by the invention is simple, the germinating time of the eel grass seeds is short, the germinating rate is high, the growth speed is high, in addition, the economic cost is low, and great significance is realized on the fast breeding and large-scale production seedling culture of the eel grass.

Description

technical field [0001] The invention relates to a method for promoting the germination and growth of bitter grass seeds. Background technique [0002] Vallisneria natana (Vallisneria natana), commonly known as noodle grass, flat shoulder grass, water leek, etc., is a perennial submerged plant of the family Hydrochardaceae, and is one of the most common submerged plants in my country. At present, the pollution caused by extensive industrial expansion to rivers, lakes and other water bodies has become increasingly prominent, and the restoration of submerged vegetation has become the key to the control of eutrophication in lakes and other water bodies in my country. Bittergrass has strong water purification ability and strong tolerance to water pollution. It plays a very important role in the restoration and reconstruction of aquatic ecosystems. However, due to the limitation of water quality and the biological characteristics of aquatic plants Due to its biological characteri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/00
Inventor 侯文华曹利静
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI