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Method for extracting astaxanthin ester from frozen Euphausia superba and enriching the astaxanthin ester

An Antarctic krill and astaxanthin ester technology, applied in organic chemistry and other directions, can solve the problems of high energy consumption of astaxanthin, affecting product quality, and oxidation of active ingredients, achieving unique methods, avoiding oxidation and damage, and effectively enriching set effect

Inactive Publication Date: 2012-07-25
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to provide a method for extracting and enriching astaxanthin esters from frozen Antarctic krill to solve the problems that the extraction of astaxanthin from dried shrimp consumes a lot of energy, and the drying process easily leads to the oxidation of active ingredients and affects the quality of the product.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Frozen Antarctic krill 500g (water content 80%, dry matter 20%), add 500mL of ethanol solution with a weight concentration of 90%, stir and extract for 1h, filter the extract, and combine the filtrate; let the combined filtrate stand for 1h to obtain the supernatant and Precipitate (discard the filter residue), then filter the precipitate, and combine the filtrate into the supernatant; concentrate the supernatant to 8% of the volume of the supernatant at 60°C and -0.09MPa pressure to obtain a concentrated extract; Add the same volume of n-hexane to the concentrated extract, shake it evenly to form a mixed solution, and immediately separate four layers with clear boundaries. From top to bottom, the third layer is the layer where the transparent red astaxanthin ester liquid is located (other Layers 1, 2, and 4 are very light yellow or nearly colorless), and the liquid in this layer is separated by a separatory funnel; the transparent red liquid containing astaxanthin ester...

Embodiment 2

[0028] Add 500g of frozen Antarctic krill (water content 80%, dry matter 20%), add 650mL of 100% absolute ethanol, stir and extract for 3 hours, filter the extract, and combine the filtrate; let the combined filtrate stand for 3 hours to obtain the supernatant and the precipitate (discard the filter residue), then filter the precipitate, and combine the filtrate into the supernatant; concentrate the supernatant to 12% of the volume of the supernatant at 65°C and -0.07MPa pressure to obtain a concentrated extract ; Add the same volume of n-hexane to the concentrated extract, shake it evenly to form a mixed solution, immediately separate four layers with clear boundaries, and the third layer from top to bottom is the layer where the transparent red astaxanthin ester liquid is located ( The other 1st, 2nd, 4th layers are very light yellow or nearly colorless), and the liquid in this layer is separated by a separatory funnel; the transparent red liquid containing astaxanthin ester ...

example example 3

[0030] Add 500g of frozen Antarctic krill (water content 80%, dry matter 20%), add 600mL of ethanol solution with a weight concentration of 95%, stir and extract for 2 hours, filter the extract, and combine the filtrate; let the combined filtrate stand for 3 hours to obtain the supernatant Liquid and sediment (discard the filter residue), then filter the precipitate, and combine the filtrate into the supernatant; concentrate the supernatant to 12% of the volume of the supernatant at 65°C and -0.07MPa pressure to obtain concentrated extraction solution; add the same volume of n-hexane to the concentrated extract, shake it evenly to form a mixed solution, and immediately separate four layers with clear boundaries, the third layer from top to bottom is the layer where the transparent red astaxanthin ester liquid is located (Other layers 1, 2, and 4 are very light yellow or nearly colorless), separate the liquid in this layer with a separatory funnel; evaporate the transparent red ...

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PUM

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Abstract

The invention provides a method for extracting astaxanthin ester from frozen Euphausia superba and enriching the astaxanthin ester. The method comprises the following steps: a) feeding Euphausia superba and ethanol into an extracting container at the ratio of 1 kilogram of frozen Euphausia superba to 1-1.2 liters of 90-100% ethanol solution, agitating and extracting for 4-6 times at normal temperature with each time for 1-3h, filtering extracted liquid, and combining the filtrates; b) standing and precipitating the combined filtrate of step a) to obtain supernatant and precipitated liquid; c) concentrating the supernatant of step b) to obtain concentrated extracted liquid; d) adding an equivalent volume of n-hexane into the concentrated extracted liquid of step c), mixing uniformly, standing for delamination to form four obvious layers, and separating transparent and red liquid on a third layer from the top to the bottom; and e) concentrating the transparent and red liquid of step d) into 1 / 4-1 / 5 of the original volume at temperature of 50 to 55 DEG C and pressure of -0.07 to -0.09MPa to obtain the product.

Description

technical field [0001] The invention belongs to the technical scope of extraction of chemical and pharmaceutical products, and particularly relates to a method for extracting and enriching astaxanthin ester from frozen Antarctic krill for food, medicine, health food, chemicals and the like. Background technique [0002] Antarctic krill (Euphausia superba), also known as giant krill or Antarctic krill, is a type of krill that lives in the waters of Antarctica in the Southern Ocean. Live in clusters, sometimes with a density of 10,000-30,000 / m 3 . They are probably the most successful animal species on Earth, at about 500 million tons. The Antarctic krill fishery is approximately 100,000 tonnes per year. The main fishing countries are Japan and Poland. The product is mainly used as cooking in Japan, while in other parts of the world it is used as animal feed and fishing bait. [0003] More than 90% of natural astaxanthin exists in the form of esterification, which is rela...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C403/24
Inventor 刘斐刘代成
Owner SHANDONG NORMAL UNIV