Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for producing yeast extract by utilizing anaerobism of tetracycline bacteria residue

A technology of yeast extract and tetracycline, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, and microbial dissolution, etc., can solve the problems of high price, high production cost of yeast extract, and difficulty in achieving practical and affordable applications. , to achieve the effect of low production cost, increase product competitiveness and low price

Inactive Publication Date: 2012-07-25
NINGXIA UNIVERSITY
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the raw materials for the production of yeast extract are mainly grain crops such as wheat as raw materials, the price is relatively high, so the production cost of yeast extract is still at a relatively high level, and it is difficult to achieve practical and affordable applications.
[0004] At present, there is no method for the anaerobic production of yeast extract using tetracycline slag

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for anaerobically producing yeast extract by using tetracycline scum, the method comprising the steps of:

[0032] a. Seed activation culture: Pick a ring from the storage tubes of Saccharomyces cerevisiae and EM original dew, respectively, insert them into the seed activation medium, culture anaerobically at 35°C for 48 hours, and obtain activated Saccharomyces cerevisiae and EM original dew seeds respectively;

[0033] b. First-level seed preparation: Inoculate the Saccharomyces cerevisiae and EM raw dew seeds prepared in the above step a with an inoculation amount of 10% by mass percentage in the seed expansion medium, and culture them anaerobically at 35°C for 48 hours to obtain Saccharomyces cerevisiae and EM primary seeds;

[0034] c. Secondary seed preparation: Inoculate the Saccharomyces cerevisiae prepared in the above step b and the primary seed of EM original dew as 10% by mass percentage in the seed expansion medium respectively, and anaerobically ...

Embodiment 2

[0039] A method for anaerobically producing yeast extract by using tetracycline scum, the method comprising the steps of:

[0040] a. Seed activation culture: Pick a ring from the storage tubes of Saccharomyces cerevisiae and EM original dew, respectively, insert them into the seed activation medium, culture anaerobically at 30°C for 72 hours, and obtain activated Saccharomyces cerevisiae and EM original dew seeds respectively;

[0041]b. First-level seed preparation: Inoculate the Saccharomyces cerevisiae and EM raw dew seeds prepared in the above step a with an inoculum amount of 5% by mass percentage, respectively, inoculate in the seed expansion medium, and culture anaerobically at 30°C for 72 hours to obtain Saccharomyces cerevisiae and EM primary seeds;

[0042] c. Secondary seed preparation: inoculate the Saccharomyces cerevisiae prepared in the above step b and the primary seed of EM original dew with an inoculation amount of 5% by mass percentage, respectively, in th...

Embodiment 3

[0047] A method for anaerobically producing yeast extract by using tetracycline scum, the method comprising the steps of:

[0048] a. Seed activation culture: Pick a ring from the storage tubes of Saccharomyces cerevisiae and EM original dew, respectively, insert them into the seed activation medium, and culture anaerobically at 25°C for 96 hours to obtain activated Saccharomyces cerevisiae and EM original dew seeds respectively;

[0049] b. First-level seed preparation: Inoculate the Saccharomyces cerevisiae and EM raw dew seeds prepared in the above step a with an inoculation amount of 1% by mass percentage, respectively, inoculate the seed expansion medium, culture anaerobically at 25°C for 96 hours, and obtain Saccharomyces cerevisiae and EM primary seeds;

[0050] c. Secondary seed preparation: Inoculate the Saccharomyces cerevisiae prepared in the above step b and the primary seed of EM original dew with an inoculation amount of 1% according to the mass percentage, resp...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for utilizing tetracycline bacteria residue, specifically to a method for producing yeast extract by utilizing anaerobism of tetracycline bacteria residue, which is characterized in that the method comprises the following steps: a, processing seeds activation and cultivation; b, processing first-grade seeds preparation; c, processing second-grade seeds preparation; d, processing bacteria culture medium preparation; e, inoculating brewing yeast and EM original-dew second-grade seeds prepared by the step c into the bacteria culture medium prepared by the step d according to mass percent of 1-10% of inoculation amount; and processing steps such as anaerobic fermentation, enzymolysis, centrifugation, micro-filtering membrane filtration, concentration and the like so as to obtain the yeast extract. According to The invention, a plenty of waste bacteria residue discharged by tetracycline production enterprises can be transformed in high efficiency; simultaneously, products thereof can be widely used for fields such as biological fermentation, bioengineering, biomedical and the like; in addition, technology is simple, and cost is low.

Description

technical field [0001] The invention relates to a method for utilizing tetracycline scum, in particular to a method for anaerobically producing yeast extract by using tetracycline slag. Background technique [0002] China is a big producer of tetracycline antibiotics, and its annual output ranks first in the world. However, in the production process of tetracycline, a large amount of waste bacteria residues will be produced. These bacteria residues are mainly the bacteria cakes formed when the fermentation mash is separated from the solid and liquid. The main components are microbial mycelium, unmetabolized organic matter, Inorganic salt etc. For a long time, antibiotic residues are mostly used as animal feed or fertilizer after simply drying or drying, and the economic added value is low. More importantly, the fungus residue will emit a special odor during the drying or drying process. Moreover, the fungus residue is easy to cause secondary fermentation due to its high co...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/06C12R1/865
Inventor 马玉龙王敏惠兴育马志强
Owner NINGXIA UNIVERSITY