Method for extracting quercetin from eucommia leaves
A technology of quercetin and eucommia leaves, which is applied in the field of extracting quercetin from eucommia leaves, can solve the problems of numerous procedures, complex processes, and high costs, and achieve the effects of easy popularization and application, simple processes, and convenient operation
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preparation example Construction
[0018] Preparation of Reference Substance Solution Accurately weigh the quercetin reference substance dried overnight with phosphorus pentoxide, add methanol to make a solution containing 30 μg per 1 mL, and obtain it.
[0019] Preparation of the test solution Take about 1 g of eucommia leaf powder, weigh it accurately, put it in a Soxhlet extractor, add chloroform to reflux and extract for 2 hours, discard the solvent, evaporate the solid part to dryness, add methanol to reflux and extract for 4 hours, Evaporate the extract to dryness, add methanol-25% hydrochloric acid solution (4:1) mixture 25mL to the residue, heat to reflux for 30 minutes, let cool, transfer to a 50mL measuring bottle, add methanol to the mark, shake well, and obtain.
[0020] Determination method Precisely draw 10 μL each of the reference substance solution and the test solution, inject it into the liquid chromatograph, measure and calculate the content of quercetin.
Embodiment 1
[0021] Example 1, a method for extracting quercetin from leaves of Eucommia ulmoides, remove impurities and pulverize leaves of Eucommia ulmoides, pass through a 40-mesh sieve, take 1Kg, add 10L of 80% ethanol solution to reflux for extraction for 1.5h, filter the liquid, and refill the solid part 8L of 70% ethanol solution was extracted for 1.5h, and the resulting extract was decompressed to recover the ethanol solution to obtain a concentrated solution; 4L of water was added to the concentrated solution for dispersion, and then 1L of D-101 macroporous adsorption resin column was added for adsorption, with a flow rate of 3L / h. After the end, eluted with 12BV of deionized water and 5BV of 70% ethanol in sequence, collected the ethanol eluate, recovered ethanol under reduced pressure, added sulfuric acid with a mass concentration of 2% to the concentrated solution to adjust the pH to 2, heated to 80°C for hydrolysis, The hydrolyzed solution was concentrated to 1 / 5 volume, and fi...
Embodiment 2
[0022] Example 2, a method for extracting quercetin from eucommia leaves, removing impurities and pulverizing eucommia leaves, passing through a 40-mesh sieve, taking 1Kg, adding 12L of 90% ethanol solution for reflux extraction for 1.5h, suction filtering the liquid, and adding the solid part 10L of 80% ethanol solution was extracted for 1.5h, and the extract was decompressed to recover the ethanol solution. After the concentrate was dispersed with 5L of water, add 1L of NKA-II macroporous adsorption resin column for adsorption, flow rate 2.5L / h, after the adsorption was completed, eluted with 10BV of deionized water and 8BV of 60% ethanol in sequence, collected the ethanol eluate, and reduced Pressure recovery ethanol. Add hydrochloric acid with a mass concentration of 4% to the obtained concentrated solution to adjust the pH of the solution to 3, heat to 90°C for hydrolysis, the hydrolyzed solution is concentrated to 1 / 5 volume, and suction filtered, the solid is 9.1 g of c...
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