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Method for preparing biodiesel from unicellular bacterial lipase by catalysis

A biodiesel and catalytic preparation technology, applied in the direction of fatty acid esterification, biofuel, bulk chemical production, etc., can solve the problems of environmental pollution, large amount, cumbersome post-treatment of reaction liquid, etc., and achieve simplification, separation and purification of subsequent processing simplified effect

Inactive Publication Date: 2012-09-19
雷菊花
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The traditional transesterification reaction uses a homogeneous catalyst, such as NaOH, KOH, sodium methylate, etc., and the dosage is about 1% based on vegetable oil. The reaction temperature is generally the boiling point of methanol, and the reaction time is 1 hour. The conversion rate is high, reaching more than 95%. But there are also obvious disadvantages at the same time. Although the reaction of alkali transesterification is simple and convenient, in order to remove the alkali catalyst, the post-treatment of the reaction solution is very complicated, and the product needs to be neutralized and washed, which will bring a large amount of industrial wastewater and cause environmental pollution.

Method used

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  • Method for preparing biodiesel from unicellular bacterial lipase by catalysis

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Experimental program
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Effect test

Embodiment 1

[0011] In a 1L autoclave with magnetic stirring, add 100.0 grams of crude linseed oil and 146.7 grams of industrial grade methanol respectively, the molar ratio of alcohol to oil is 42~21:1 (based on linseed oil) single enzyme catalyst 0.05%, dissolve in 3ml methanol , stirred and heated to 125±5°C and the pressure was 6.9-7.0Mpa, and the reaction was completed after 120 minutes of timing reaction, excess methanol was distilled, and the reaction solution was poured into a separatory funnel while it was hot and allowed to stand for stratification. The lower layer was crude biodiesel. The upper layer is the by-product crude glycerin, the pH value of the upper and lower layers is 6-7, and samples were taken separately, and the analysis results are listed in the table below. The pH value of the crude product of the upper and lower layers of the reaction is 6-7, so no post-treatment is required, avoiding the usual process of acid neutralization and water washing in the alkali-lipid ...

Embodiment 2

[0013] Change the single-enzyme catalyst addition into 0.03% in the above-mentioned embodiment 1, other conditions are the same, and the test results are listed in the table. The pH value of crude glycerol, a by-product of the upper layer reaction, is 7-8, and the pH value of crude biodiesel, a reaction product of the lower layer, is 6-7, so there is no need for post-treatment. The content of methyl ester detected by chromatography is 99.1%, and the yield of biodiesel is as high as 99.7%.

Embodiment 3

[0015] The alcohol molar ratio of above-mentioned embodiment 1 is reduced to 21: 1 other conditions are identical, and test result is listed in the table. The pH value of the crude product of the reaction in the upper and lower layers is 6-7, and there is still no need for post-treatment. The content of methyl ester detected by chromatography is 95.1%, and the yield of biodiesel is as high as 97.3%.

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Abstract

The invention discloses a method for preparing biodiesel from unicellular bacterial lipase by catalysis. The invention belongs to a new technology for preparing biomass energy. Trace amounts of the unicellular bacterial lipase is added into kinds of vegetable oils, and enzymic catalytic reaction is carried out with a catalytic method, so that the crude product biodiesel and by-product glycerol are neutral without neutralization and water washing, and post-treatment procedure is greatly simplified. No wastewater is discharged, so that no pollution on the environment is generated; the reaction temperature and pressure are lower than those of a supercritical technique, so that the method has the advantages of shorter heating time, higher methanol content, lower energy consumption, simpler procedure, lower process cost and easiness of industrialization. The pressure requirements on a reaction device are decreased while the yield of methyl ester can be up to 99-97%, and the prepared biodiesel product accords with main performance indexes of 0# diesel oil.

Description

technical field [0001] The invention belongs to a new technology for preparing biomass energy, in particular to a method for preparing biodiesel by catalysis of single-cell bacterial lipase. Background technique [0002] Biodiesel is a renewable and clean energy that replaces petrochemical diesel. Vegetable oil, waste gutter oil, etc. can be used as raw materials for transesterification with methanol, ethanol, etc. The product is fatty acid methyl ester (hereinafter referred to as methyl ester), and the by-product is glycerin. The traditional transesterification reaction uses a homogeneous catalyst, such as NaOH, KOH, sodium methylate, etc., and the dosage is about 1% based on vegetable oil. The reaction temperature is generally the boiling point of methanol, and the reaction time is 1 hour. The conversion rate is high, reaching more than 95%. But there are also obvious disadvantages at the same time. Although the alkali transesterification method is simple and convenient, i...

Claims

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Application Information

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IPC IPC(8): C11C3/10C12P7/64C10L1/02
CPCY02E50/13Y02E50/10Y02P20/54
Inventor 韩德民雷菊花
Owner 雷菊花