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Preparation method of plant polysaccharide sulfate with immune enhanced activity and anti-virus effect, as well as application

An immune-enhancing, plant polysaccharide technology, applied in the direction of antiviral agents, applications, allergic diseases, etc., can solve problems such as no biological activity or biological activity

Active Publication Date: 2015-01-21
成都市坤宏动物药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, plant polysaccharides do not have certain biological activities or their biological activities are very weak, thus limiting their application.

Method used

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  • Preparation method of plant polysaccharide sulfate with immune enhanced activity and anti-virus effect, as well as application
  • Preparation method of plant polysaccharide sulfate with immune enhanced activity and anti-virus effect, as well as application
  • Preparation method of plant polysaccharide sulfate with immune enhanced activity and anti-virus effect, as well as application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Preparation of Chuanmingshen polysaccharide: Weigh 300g of Chuanmingshen dry powder and place it in a Soxhlet extractor, heat and reflux with a 1:1 (V / V) mixture of ethanol and ether to degrease for 6 hours, filter the dregs in Dry at room temperature. Add distilled water to the medicinal residues at a material-to-liquid ratio of 1:20 (g / ml), extract 3 times at 100°C, 3 hours each time, combine the extracts, concentrate to a small volume, dialyze with running water for 48 hours, and then dialyze with distilled water for 24 hours. The volume of chloroform is 1 / 5 of the extract solution, then add n-butanol with a volume of 1 / 5 of chloroform, shake vigorously for 20 minutes, centrifuge to remove the denatured protein at the junction of the water layer and the organic layer, the water layer is still Repeat this method to remove protein 5 times. After concentration, add 3 times the amount of 95% ethanol (V / V) and let stand overnight. The precipitate was collected by centri...

Embodiment 2

[0018] Preparation of Chuanming ginseng polysaccharide: Weigh 200g of Chuanming ginseng dry powder and place it in a Soxhlet extractor, heat and reflux with a 1:1 (V / V) mixture of ethanol and ether to degrease for 4 hours, filter and place the dregs in Dry at room temperature. Add distilled water to the medicinal residues according to the ratio of material to liquid 1:15 (g / ml), extract twice at 90°C, 2 hours each time, combine the extracts, concentrate to a small volume, dialyze with running water for 36 hours, then dialyze with distilled water for 18 hours, and dialyze in the bag Add chloroform with a volume of 1 / 5 of the extract, then add n-butanol with a volume of 1 / 5 of chloroform, shake vigorously for 15 min, and centrifuge to remove denatured proteins at the junction of the water layer and the organic layer. The layer was still removed protein 3 times according to this method. After concentration, add 2 times the amount of 95% ethanol (V / V) and let stand overnight. Th...

Embodiment 3

[0022] Preparation of Chuanming ginseng polysaccharide: Weigh 100g of Chuanming ginseng dry powder and put it in a Soxhlet extractor, heat and reflux with a 1:1 (V / V) mixture of ethanol and ether to degrease for 4 hours, filter and place the dregs in Dry at room temperature. Add distilled water to the medicinal dregs according to the ratio of material to liquid 1:10, extract twice at 100°C for 1.5 hours each time, combine the extracts, concentrate to a small volume, dialyze with running water for 24 hours, and then dialyze with distilled water for 12 hours. The volume of the liquid in the dialysis bag is Add 1 / 5 chloroform to the extract, then add n-butanol whose volume is 1 / 5 chloroform, shake vigorously for 10 minutes, and centrifuge to remove the denatured protein at the junction of the water layer and the organic layer. This method was repeated 4 times to remove protein. After concentration, 1.5 times the amount of 95% ethanol (V / V) was added and allowed to stand overnigh...

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Abstract

The invention discloses a plant polysaccharide sulfate with immune enhanced activity and anti-virus effect and a preparation method thereof. The preparation method comprises the following steps of: extracting and refining polysaccharide from a Chinese medicament Chuanminshen violaceum; uniformly distributing Chuanminshen violaceum polysaccharide in anhydrous formamide; adding an esterification reagent; and preparing the Chuanminshen violaceum polysaccharide sulfate at a certain temperature. The prepared Chuanminshen violaceum polysaccharide sulfate has immune enhanced activity, in-vitro activity resistant to newcastle disease virus and duck plague virus, and can be used as a biological feed additive which resists diseases, promotes growth and does not have residues in livestock.

Description

technical field [0001] The invention relates to a preparation method and application of a plant polysaccharide sulfate compound with immune enhancing activity and antiviral effect, in particular to a biological feed additive Chuan Mingshen polysaccharide sulfate ester compound with immune enhancing activity and antiviral activity and its Preparation method and application. Background technique [0002] Drug feed additives play an important role in my country's livestock and poultry breeding industry because of their functions of promoting the growth of livestock and poultry and preventing diseases, improving productivity and raising economic benefits. However, with the long-term use of pharmaceutical feed additives, especially the abuse of antibiotics and chemically synthesized drugs in feed, problems such as drug resistance, drug residues, and environmental pollution have emerged. This not only reduces the quality of livestock products, but also affects human health and ec...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P31/12A61P37/04A23K1/16
Inventor 殷中琼贾仁勇程安春宋旭徐娇
Owner 成都市坤宏动物药业有限公司
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