Continuous gradient mixing device and mixing method thereof

Abstract

The invention discloses a continuous gradient mixing device, which comprises a first container for accommodating a low-concentration solution, a second container for accommodating a high-concentration solution, a third container for accommodating a gradient mixing solution, a first piston cylinder body and a second piston cylinder body, wherein a communicating pipeline extending into the first container is arranged at the bottom of the first piston cylinder body; a communicating pipeline extending into the second container and a communicating pipeline communicated to the third container are arranged at the bottom of the second piston cylinder body; the first piston cylinder body is communicated with the second piston cylinder body through a unidirectional communicating pipeline; the unidirectional communicating pipeline comprises a pipeline inlet and a pipeline outlet; and an included angle between the pipeline outlet and the perpendicular direction is 0 to 80 degrees. The invention also discloses a method for performing gradient mixing by the continuous gradient mixing device. Due to the basic theory of fluid mechanics, liquid can be instantly and uniformly mixed in the containers; the mixing effect is uniform and continuous; and large-scale production can be reliably guaranteed.

Description

Technical field: [0001] The invention relates to a mixing device for pouring and producing polyacrylamide gradient gel in the biological field, in particular to a continuous gradient mixing device and a mixing method for producing polyacrylamide gradient gel and laboratory pouring gradient gel. Background technique: [0002] Gradient gel electrophoresis usually uses polyacrylamide gels, but instead of running on a single concentration (pore size) gel, gradient gels are formed. The concentration of acrylamide varies from the top of the gel to the bottom of the gel, such as usually 5% for the top gel and 25% for the bottom gel. [0003] The gel gradient is formed by a special gradient mixing device. The high-concentration acrylamide solution is first added to the glass plate, and then the concentration of the solution decreases in a gradient, so the pore size at the top of the gel is larger, and the pore size at the bottom of the gel is larger. smaller. Gradient gel electrop...

AI Technical Summary

Problems solved by technology

It mainly uses gravity to make two different liquids enter the same container, and then mix them under the action of mechanical stirring. The disadvantage of this method is that the mixing of liquids in the B cup is not completely linear, and the mixing effect is not ideal. , the obtained curve is as attached figure 2 Curves that deviate from theory in the upper middle

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