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Zebra fish embryo breeding liquid and preparation method thereof

A zebrafish embryo and zebrafish technology are applied in the field of zebrafish embryo culture fluid and its preparation, and can solve the problem of lack of zebrafish culture fluid and the like

Inactive Publication Date: 2012-10-10
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, there is no announcement of zebrafish culture medium. The present invention solves the problem of selection of culture medium in zebrafish embryo culture, and provides a culture medium capable of ensuring the survival rate and survival state of zebrafish and its preparation method

Method used

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  • Zebra fish embryo breeding liquid and preparation method thereof
  • Zebra fish embryo breeding liquid and preparation method thereof
  • Zebra fish embryo breeding liquid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Preparation of Embodiment 1 Culture Solution 1

[0024] Weigh analytically pure 17.5g of NaCl, 0.76g of KCl, 2.91g of CaCl by weight 2 , 2.38g of MgSO 4 , and then dissolved with 300ml of pure water, then added 0.5ml of methylene blue, and then added pure water to make up the volume of the culture solution to 1000ml, to obtain zebrafish culture solution 1.

Embodiment 2

[0025] The preparation of embodiment 2 culture solution 2

[0026] Weigh analytically pure 17.0g of NaCl, 0.80g of KCl, 2.8g of CaCl by weight 2 , 2.4g of MgSO 4 , and then dissolved with 200ml of pure water, then added 0.6ml of methylene blue, and then added pure water to make up the volume of the culture solution to 1000ml, to obtain zebrafish culture solution 2.

Embodiment 3

[0027] The preparation of embodiment 3 culture solution 3

[0028] Weigh analytically pure 18.0g of NaCl, 0.70g of KCl, 2.7g of CaCl by weight 2 , 2.9g of MgSO 4 , and then dissolved with 250ml of pure water, then added 0.4ml of methylene blue, and then added pure water to make up the volume of the culture solution to 1000ml, to obtain the zebrafish culture solution 3.

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Abstract

The invention relates to zebra fish embryo breeding liquid and a preparation method thereof. The breeding liquid is composed of sodium chloride, potassium chloride, calcium chloride, magnesium sulfate, methylene blue and pure water. The breading liquid simulates a water environment of zebra fish in tropical seawater, and is prepared to meet the requirements of a laboratory, and an indicator is added into the breeding liquid for facilitating dual effects of biological experiments and growing and breeding of the zebra fish.

Description

technical field [0001] The invention belongs to the field of culture solution preparation, in particular to a zebrafish embryo culture solution and a preparation method thereof. Background technique [0002] Zebrafish is another important model organism after the mouse in the 21st century. It has many characteristics such as strong reproductive ability, in vitro fertilization and development, transparent embryo, short sexual maturation cycle, small individual and easy breeding. The cardiovascular, blood, digestive tract, liver, and vision of zebrafish share many features with humans. [0003] The zebrafish genome contains about 30,000 gold genes, which is about the same as that of a human, and many of its genes have a corresponding relationship with the human body. Due to the similarity of genes and development mechanisms between humans and fish, zebrafish has become an excellent material for studying animal embryonic development in recent years. [0004] In addition, zebr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/073
Inventor 陈峰
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI